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PMID: 231766 Published · ppublish English Journal Article

New genes and promoters suggested by the DNA sequence near the end of the coliphage T7 early operon.

Nucleic acids research ·Vol. 7 ·No. 7 ·1979-12-11 ·Pages 1931-43

Boothroyd JC, Hayward RS

Abstract

We have employed the dideoxynucleotide chain-terminating method to determine the nucleotide sequence of T7 DNA between the physical map positions 18.9% and 19.8%. The most striking features of this sequence are two perfect 21-basepair repeats, each of which appears to contain a promoter for late transcription. In each case the promoter sequence incorporates a putative translational terminator on its left (5'-side of the "sense" strand), and overlaps a potential ribosome-binding site on its right. The region probably lies immediately distal to the early operon, and may contain two short, hitherto unreported protein-coding sequences.

MeSH Terms
Base Sequence Chromosome Mapping DNA Restriction Enzymes DNA, Viral/genetics Genes, Viral Operon Protein Biosynthesis T-Phages/genetics Time Factors Transcription, Genetic
Chemicals
DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Boothroyd J C
Hayward R S
References (32)
32 references, click to expand
  1. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  2. The size and messenger RNA activity of bacteriophage T7 late transcripts synthesized in vivo.
    J Mol Biol. 1978 Jun 15;122(1):69-101 PMID: 671552
  3. Sequences and efficiencies of proposed mRNA terminators.
    Nature. 1978 Jan 19;271(5642):275-7 PMID: 622168
  4. Two ribosome binding sites from the gene 0-3 messenger RNA of bacteriophages T7.
    J Mol Biol. 1977 Aug 25;114(4):527-43 PMID: 915941
  5. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
    J Mol Biol. 1977 Feb 15;110(1):119-46 PMID: 845942
  6. Dinucleotide sequences in the regions of T7 DNA coding for termination of early transcription.
    Eur J Biochem. 1974 Oct 1;48(1):199-208 PMID: 4614973
  7. Transcriptional termination in vitro: the 3'-terminal sequence of coliphage T7 "early" RNA.
    Biochem Biophys Res Commun. 1974 Nov 27;61(2):809-16 PMID: 4616695
  8. Transcription of the early region of bacteriophage T7: selective initiation with dinucleotides.
    J Mol Biol. 1973 Jun 25;77(2):255-77 PMID: 4587752
  9. A preliminary map of the major transcription units read by T7 RNA polymerase on the T7 and T3 bacteriophage chromosomes.
    Proc Natl Acad Sci U S A. 1974 Mar;71(3):760-4 PMID: 4522790
  10. Physical mapping of the early region of bacteriophage T7 DNA.
    J Mol Biol. 1973 Sep 15;79(2):249-65 PMID: 4586410
  11. Characterization of T7-specific ribonucleic acid polymerase. 1. General properties of the enzymatic reaction and the template specificity of the enzyme.
    J Biol Chem. 1973 Mar 25;248(6):2235-44 PMID: 4570474
  12. Length measurements of RNA synthesized in vitro by Escherichia coli RNA polymerase.
    J Mol Biol. 1973 Mar 15;74(4):677-87 PMID: 4354077
  13. Improved estimation of secondary structure in ribonucleic acids.
    Nat New Biol. 1973 Nov 14;246(150):40-1 PMID: 4519026
  14. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.
    Biochemistry. 1973 Jul 31;12(16):3055-63 PMID: 4354250
  15. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
  16. Bacteriophage T7.
    Science. 1972 Apr 28;176(4033):367-76 PMID: 4554613
  17. New RNA polymerase from Escherichia coli infected with bacteriophage T7.
    Nature. 1970 Oct 17;228(5268):227-31 PMID: 4920917
  18. How ribosomes select initiator regions in mRNA: base pair formation between the 3' terminus of 16S rRNA and the mRNA during initiation of protein synthesis in Escherichia coli.
    Proc Natl Acad Sci U S A. 1975 Dec;72(12):4734-8 PMID: 1107998
  19. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.
    Biochemistry. 1975 Aug 26;14(17):3787-94 PMID: 1174504
  20. Recognition and initiation site for four late promoters of phage T7 is a 22-base pair DNA sequence.
    Nature. 1979 Jul 5;280(5717):35-9 PMID: 15305578
  21. Restriction and modification enzymes and their recognition sequences.
    Gene. 1978 Nov;4(3):183-94 PMID: 369952
  22. The DNA sequence at the T7 C promoter.
    Nucleic Acids Res. 1979 Feb;6(2):525-44 PMID: 370790
  23. T7 RNA polymerase: promoter structure and polymerase binding.
    Biochemistry. 1979 Feb 6;18(3):528-37 PMID: 369603
  24. Mapping of class II promoter sites utilized in vitro by T7-specific RNA polymerase on bacteriophage T7 DNA.
    J Virol. 1979 Jan;29(1):196-208 PMID: 430592
  25. Nucleotide sequence of the three major early promoters of bacteriophage T7.
    Nucleic Acids Res. 1979;6(5):1895-907 PMID: 377234
  26. Four T7 RNA polymerase promoters contain an identical 23 bp sequence.
    Cell. 1979 Apr;16(4):815-25 PMID: 455451
  27. Structure of a promoter for T7 RNA polymerase.
    Proc Natl Acad Sci U S A. 1977 Oct;74(10):4266-70 PMID: 270669
  28. The relationship between function and DNA sequence in an intercistronic regulatory region in phage lambda.
    Nature. 1978 Mar 30;272(5652):414-23 PMID: 634366
  29. An expanded transcriptional map of T7 bacteriophage. Reading of minor T7 promoter sites in vitro by Escherichia coli RNA polymerase.
    J Mol Biol. 1977 Jun 5;112(4):577-601 PMID: 875034
  30. Kinetics of initiation of bacterial protein synthesis.
    Proc Natl Acad Sci U S A. 1979 Jan;76(1):251-5 PMID: 370825
  31. A rho-dependent termination site in the gene coding for tyrosine tRNA su3 of Escherichia coli.
    Nature. 1978 Mar 30;272(5652):423-8 PMID: 345126
  32. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/7.7.1931
Published
1979-12-11
Pages
1931-43
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342357
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/7.7.1931 PMC Full Text View journal details
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