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PMID: 370790 Published · ppublish English Journal Article

The DNA sequence at the T7 C promoter.

Nucleic acids research ·Vol. 6 ·No. 2 ·1979-02-00 ·Pages 525-44

McConnell DJ

Abstract

Restriction fragments of T7 DNA which selectively bind E. coli RNA polymerase have been identified. These include fragments located close to the beginning of gene 1 where according to Minkley and Pribnow (1973) there is a promoter called C. The smallest fragment from this region which binds RNA polymerase has been sequenced. It contains a promoter-like sequence, at an appropriate distance from the sequence TACA which Minkley and Pribnow suggested should lie at the initiation site of C. RNA synthesised in vitro from these fragments has been sequenced. The RNA sequence corresponds to the sequence to the right of the C promoter. The C promoter differs significantly from the A1 A2 and A3 promoters in sequence. Its structure and position suggest it plays a role in T7 infection.

MeSH Terms
Base Sequence Coliphages/analysis DNA Restriction Enzymes DNA, Viral DNA-Directed RNA Polymerases/metabolism Escherichia coli/enzymology Transcription, Genetic
Chemicals
DNA, Viral DNA-Directed RNA Polymerases DNA Restriction Enzymes
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
McConnell D J
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38 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/6.2.525
Published
1979-02-00
Pages
525-44
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327711
Subset
IM
Databases
GENBANK
J02518
External Links
PubMed source DOI: 10.1093/nar/6.2.525 PMC Full Text View journal details
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