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PMID: 232228 Published · ppublish English Journal Article

Transposon 10 promoted deletions and inversions in the transfer genes of R100-1.

Molecular & general genetics : MGG ·Vol. 176 ·No. 1 ·1979-10-02 ·Pages 113-20

Kehoe MA, Foster TJ

Abstract

Spontaneous tetracycline-sensitive, transfer-deficient mutants of R100-1 were selected and analysed by genetic complementation tests and with the restriction endonuclease EcoR1. While some of the Tets Tra- mutants were caused by a single deletion event which removed the Tetr genes and extended into the neighbouring transfer genes, other mutants were the result of the deletion of the Tetr genes within Tn10 which was accompanied by an inversion of adjacent DNA sequences. A clustering of deletion and inversion endpoints occurred in the traA gene. Some of the transfer genes of R100-1 were assigned to EcoR1 fragments.

MeSH Terms
Chromosome Deletion Chromosome Inversion Conjugation, Genetic DNA Restriction Enzymes DNA Transposable Elements Escherichia coli/genetics Genes Genetic Complementation Test Mutation R Factors Tetracycline
Chemicals
DNA Transposable Elements DNA Restriction Enzymes Tetracycline
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kehoe M A
Foster T J
References (25)
25 references, click to expand
  1. Genetic analysis of transfer by the Escherichia coli sex factor F, using P1 transductional complementation.
    J Bacteriol. 1972 Jun;110(3):843-51 PMID: 4555413
  2. R factor-mediated tetracycline resistance in Escherichia coli K12. Dominance of some tetracycline sensitive mutants and relief of dominance by deletion.
    Mol Gen Genet. 1976 Feb 2;143(3):339-44 PMID: 765766
  3. Molecular cloning of DNA from F sex factor of Escherichia coli K-12.
    Proc Natl Acad Sci U S A. 1976 Jan;73(1):64-8 PMID: 1108020
  4. Plasmid co-integrates of prophage lambda and R factor R100.
    J Bacteriol. 1976 Apr;126(1):166-76 PMID: 770421
  5. Genetic analysis of deletions of R100-1 that are both transfer-deficient and tetracycline-sensitive.
    J Gen Microbiol. 1976 Mar;93(1):133-40 PMID: 772162
  6. Conjugational complementation analysis of transfer-deficient mutants of Flac in Escherichia coli.
    J Bacteriol. 1972 Jun;110(3):831-42 PMID: 4555412
  7. Characterization of the F transfer cistron, traL.
    Genet Res. 1973 Apr;21(2):205-13 PMID: 4574420
  8. Mutagenesis by insertion of a drug-resistance element carrying an inverted repetition.
    J Mol Biol. 1975 Oct 5;97(4):561-75 PMID: 1102715
  9. Phenotypic characterization of R-factor tetracycline resistance determinants.
    Genet Res. 1974 Dec;24(3):333-43 PMID: 4618215
  10. Transfer-defective mutants of sex factors in Escherichia coli. I. Defective mutants and complementation analysis.
    Genetics. 1970 Feb;64(2):173-88 PMID: 4919134
  11. Translocatable elements in procaryotes.
    Cell. 1977 May;11(1):11-23 PMID: 326413
  12. Tra cistrons and proteins encoded by the Escherichia coli antibiotic resistance plasmid R6-5.
    Mol Gen Genet. 1978 Jul 11;163(2):169-79 PMID: 355846
  13. Translocation of the tetracycline resistance determinant from R100-1 to the Escherichia coli K-12 chromosome.
    J Bacteriol. 1975 Dec;124(3):1153-8 PMID: 1104574
  14. Cloning and characterization of EcoRI and HindIII restriction endonuclease-generated fragments of antibiotic resistance plasmids R6-5 and R6.
    Mol Gen Genet. 1978 Jun 14;162(2):121-37 PMID: 672900
  15. Electron microscope heteroduplex studies of sequence relations among bacterial plasmids: identification and mapping of the insertion sequences IS1 and IS2 in F and R plasmids.
    J Bacteriol. 1975 May;122(2):764-75 PMID: 1092668
  16. Occurrence of insertion sequence (IS) regions on plasmid deoxyribonucleic acid as direct and inverted nucleotide sequence duplications.
    J Bacteriol. 1975 May;122(2):776-81 PMID: 1092669
  17. Transposable genetic elements and plasmid evolution.
    Nature. 1976 Oct 28;263(5580):731-8 PMID: 792710
  18. EcoRI restriction endonuclease map of the composite R plasmid NR1.
    J Bacteriol. 1976 Jul;127(1):619-36 PMID: 776943
  19. Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterization of transfer-deficient mutants.
    J Bacteriol. 1971 May;106(2):529-38 PMID: 4929865
  20. Plasmid specificity of two proteins required for conjugation in E. coli K12.
    Nat New Biol. 1971 Apr 7;230(14):183-5 PMID: 4928925
  21. Genetic analysis of mutations in the transfer genes of pDU202 tra::Tn10 plasmids, caused by the excision of Tn10.
    Mol Gen Genet. 1977 Nov 29;157(1):109-18 PMID: 340912
  22. Deletion map of the chloramphenicol resistance region of R1 and R100-1.
    J Bacteriol. 1973 Nov;116(2):1062-3 PMID: 4583232
  23. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. II. Structure of drug resistance (R) factors and F factors.
    J Mol Biol. 1973 Apr 5;75(2):235-55 PMID: 4580676
  24. Tetracycline-sensitive mutants of the F-like R factors R100 and R100-1.
    Mol Gen Genet. 1975;137(1):85-88 PMID: 1101030
  25. Identification and characterization of four new tra cistrons on the E. coli K12 sex factor F.
    Plasmid. 1978 Jun;1(3):316-23 PMID: 372967
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00334302
Published
1979-10-02
Pages
113-20
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00334302
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