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PMID: 23468594 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A safeguard mechanism regulates Rho GTPases to coordinate cytokinesis with the establishment of cell polarity.

PLoS biology ·Vol. 11 ·No. 2 ·2013-00-00 ·Pages e1001495

Meitinger F, Richter H, Heisel S, Hub B, Seufert W, Pereira G

Abstract

The spatiotemporal control of cell polarity is crucial for the development of multicellular organisms and for reliable polarity switches during cell cycle progression in unicellular systems. A tight control of cell polarity is especially important in haploid budding yeast, where the new polarity site (bud site) is established next to the cell division site after cell separation. How cells coordinate the temporal establishment of two adjacent polarity sites remains elusive. Here, we report that the bud neck associated protein Gps1 (GTPase-mediated polarity switch 1) establishes a novel polarity cue that concomitantly sustains Rho1-dependent polarization and inhibits premature Cdc42 activation at the site of cytokinesis. Failure of Gps1 regulation leads to daughter cell death due to rebudding inside the old bud site. Our findings provide unexpected insights into the temporal control of cytokinesis and describe the importance of a Gps1-dependent mechanism for highly accurate polarity switching between two closely connected locations.

MeSH Terms
Cell Cycle Proteins/genetics,metabolism Cell Division/genetics,physiology Cell Polarity/genetics,physiology Cytokinesis/genetics,physiology Saccharomyces cerevisiae/cytology,enzymology Saccharomyces cerevisiae Proteins/genetics,metabolism cdc42 GTP-Binding Protein, Saccharomyces cerevisiae/genetics,metabolism rho GTP-Binding Proteins/genetics,metabolism
Chemicals
Cell Cycle Proteins Saccharomyces cerevisiae Proteins cdc42 GTP-Binding Protein, Saccharomyces cerevisiae rho GTP-Binding Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Meitinger Franz
Molecular Biology of Centrosomes and Cilia Unit, DKFZ-ZMBH Alliance, German Cancer Research Center, Heidelberg, Germany.
Richter Heidi
Heisel Sabrina
Hub Birgit
Seufert Wolfgang
Pereira Gislene
Conflict of Interest

The authors have declared that no competing interests exist.

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Article Info
Journal
PLoS biology
Abbr.
PLoS Biol
ISSN
1545-7885
DOI
10.1371/journal.pbio.1001495
Published
2013-00-00
Epub
2013-00-26
Pages
e1001495
Language
English
Region
United States
NLM ID
101183755
PMCID
PMC3582507
Subset
IM
External Links
PubMed source DOI: 10.1371/journal.pbio.1001495 PMC Full Text View journal details
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