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PMID: 2406130 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A mutation in a single gene of Schizosaccharomyces pombe affects the expression of several snRNAs and causes defects in RNA processing.

The EMBO journal ·Vol. 9 ·No. 2 ·1990-02-00 ·Pages 525-34

Potashkin J, Frendewey D

Abstract

A bank of temperature sensitive (ts-) mutants of Schizosaccharomyces pombe was screened for snRNA expression mutants using an oligodeoxynucleotide that recognizes U2 RNA. One mutant with a novel phenotype was identified that has reduced steady-state levels of the spliceosomal snRNAs U1, U2, U4, U5 and U6. In addition, the mutant exhibits a temperature-dependent accumulation of aberrant U2 and U4 transcripts elongated at their 3' end. The steady-state concentration of the RNA component of RNase P is also reduced in the mutant, whereas the amount of U3 RNA, 7SL RNA, tRNA, rRNA and mRNA are the same as wild-type. Pre-mRNA, pre-tRNA and U6 RNA precursor processing are impaired in the mutant. Genetic analysis demonstrates that the snRNA defects are tightly linked to the ts- growth defect and are recessive. We have named this mutant snm1 to indicate a defect in snRNA maintenance. The data on snm1 suggest that a single trans-acting factor is essential for the maintenance of steady-state levels of several snRNAs and for proper 3' end formation of U2 and U4 RNAs.

MeSH Terms
Base Sequence Blotting, Northern Gene Expression Genes, Fungal Molecular Sequence Data Mutation Oligonucleotide Probes RNA Precursors/genetics RNA Splicing RNA, Catalytic RNA, Fungal/genetics RNA, Ribosomal/genetics RNA, Small Nuclear/genetics Restriction Mapping Ribonucleases Saccharomycetales/genetics Schizosaccharomyces/genetics,growth & development Temperature Transcription, Genetic
Chemicals
Oligonucleotide Probes RNA Precursors RNA, Catalytic RNA, Fungal RNA, Ribosomal RNA, Small Nuclear Ribonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Potashkin J
Cold Spring Harbor Laboratory, NY 11724.
Frendewey D
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55 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-02-00
Pages
525-34
Language
English
Region
England
NLM ID
8208664
PMCID
PMC551696
Subset
IM
Grants
NIGMS NIH HHS · GM10831 · United States
NIGMS NIH HHS · GM38242 · United States
External Links
PubMed source PMC Full Text View journal details
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