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PMID: 2419901 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Blood platelets express high levels of the pp60c-src-specific tyrosine kinase activity.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 4 ·1986-02-00 ·Pages 852-6

Golden A, Nemeth SP, Brugge JS

Abstract

We have examined human and rabbit blood platelets for expression of pp60c-src, the normal cellular homolog of the transforming protein of Rous sarcoma virus. pp60c-src kinase activity was determined by an immune-complex kinase assay that uses enolase as the substrate, and pp60c-src protein levels were determined by an immunoblot assay. Lysates from platelets expressed high levels of pp60c-src-specific kinase activity and pp60c-src protein compared to the levels found in other tissues. pp60c-src was also found to be one of the major proteins phosphorylated in vitro in membranes isolated from platelets. Multiple protein species other than pp60c-src were also phosphorylated on tyrosine in the membrane phosphorylation reactions, and phosphotyrosine represented approximately equal to 80% of the total phosphoamino acid residues phosphorylated in the membranes. These results indicate that tyrosine kinases represent the major protein phosphorylating enzymes detected in isolated platelet membranes. Although the association of tyrosine kinase activity with many viral oncogene products and cellular growth hormone receptors has suggested a role for these enzymes in the regulation of cell proliferation, these results indicate that the expression of high levels of tyrosine kinase activity is not exclusively associated with proliferating cells.

MeSH Terms
Animals Blood Platelets/enzymology Cell Membrane/enzymology Erythrocytes/enzymology Humans Lymphocytes/enzymology Lymphoid Tissue/enzymology Membrane Proteins/analysis Phosphopyruvate Hydratase/metabolism Phosphorylation Protein-Tyrosine Kinases/analysis Proto-Oncogene Proteins/analysis Proto-Oncogene Proteins pp60(c-src) Rabbits
Chemicals
Membrane Proteins Proto-Oncogene Proteins Protein-Tyrosine Kinases Proto-Oncogene Proteins pp60(c-src) Phosphopyruvate Hydratase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Golden A
Nemeth S P
Brugge J S
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41 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.4.852
Published
1986-02-00
Pages
852-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC322968
Subset
IM
Grants
NCI NIH HHS · CA28146 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.4.852 PMC Full Text View journal details
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