Properties of condensed chromatin in barley nuclei.

Planta ·Vol. 149 ·No. 1 ·1980-06-00 ·Pages 69-77

Muller A, Philipps G, Gigot C

Abstract

A method for isolation and purification of intact nuclei from barley leaves was developed and several properties of the chromatin were studied. The dense structure of the main part of the chromatin does not alter the accessibility of the DNA to nucleases. 60% of the nuclear DNA can be degraded by micrococcal endonuclease. Nevertheless the solubility of the chromatin fragments depends on the extent of nuclease digestion; solubilisation occurring only when the major part of the internucleosomal DNA was degraded (≃30% of digestion). Electron microscopic observations suggest that this was due to particularly dense organization of the chromatin "in situ". The possible physiological meaning of some of these properties are discussed.

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Muller A

Laboratoire de Virologie, Institut de Biologie Moléculaire et Cellulaire du CNRS, 15, Rue Descartes, F-67084, Strasbourg Cédex, France.

Philipps G

Gigot C

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Article Info

Journal

Planta

Abbr.

Planta

ISSN

0032-0935

DOI

10.1007/BF00386230

Published

1980-06-00

Pages

69-77

Language

English

Region

Germany

NLM ID

1250576

External Links

PubMed source DOI: 10.1007/BF00386230 View journal details

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Properties of condensed chromatin in barley nuclei.

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