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PMID: 24319056 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Global analysis of cdc14 dephosphorylation sites reveals essential regulatory role in mitosis and cytokinesis.

Molecular & cellular proteomics : MCP ·Vol. 13 ·No. 2 ·2014-02-00 ·Pages 594-605

Kao L, Wang YT, Chen YC, Tseng SF, Jhang JC, Chen YJ, Teng SC

Abstract

Degradation of the M phase cyclins triggers the exit from M phase. Cdc14 is the major phosphatase required for the exit from the M phase. One of the functions of Cdc14 is to dephosphorylate and activate the Cdh1/APC/C complex, resulting in the degradation of the M phase cyclins. However, other crucial targets of Cdc14 for mitosis and cytokinesis remain to be elucidated. Here we systematically analyzed the positions of dephosphorylation sites for Cdc14 in the budding yeast Saccharomyces cerevisiae. Quantitative mass spectrometry identified a total of 835 dephosphorylation sites on 455 potential Cdc14 substrates in vivo. We validated two events, and through functional studies we discovered that Cdc14-mediated dephosphorylation of Smc4 and Bud3 is essential for proper mitosis and cytokinesis, respectively. These results provide insight into the Cdc14-mediated pathways for exiting the M phase.

MeSH Terms
Binding Sites Cell Cycle Proteins/metabolism Chromosomal Proteins, Non-Histone/metabolism Chromosome Segregation Cytokinesis/genetics DNA Packaging Mitosis/genetics Phosphoproteins/chemistry,metabolism Phosphorylation Protein Processing, Post-Translational Protein Tyrosine Phosphatases/metabolism Proteome/analysis,metabolism Saccharomyces cerevisiae/metabolism Saccharomyces cerevisiae Proteins/chemistry,metabolism Tandem Mass Spectrometry
Chemicals
BUD3 protein, S cerevisiae CDC14 protein, S cerevisiae Cell Cycle Proteins Chromosomal Proteins, Non-Histone Phosphoproteins Proteome SMC4 protein, S cerevisiae Saccharomyces cerevisiae Proteins Protein Tyrosine Phosphatases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Kao Li
Department of Microbiology, College of Medicine, National Taiwan University, Taipei 10051, Taiwan;
Wang Yi-Ting
Chen Yu-Chen
Tseng Shun-Fu
Jhang Jia-Cin
Chen Yu-Ju
Teng Shu-Chun
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Article Info
Journal
Molecular & cellular proteomics : MCP
Abbr.
Mol Cell Proteomics
ISSN
1535-9484
DOI
10.1074/mcp.M113.032680
Published
2014-02-00
Epub
2013-00-07
Pages
594-605
Language
English
Region
United States
NLM ID
101125647
PMCID
PMC3916656
Subset
IM
External Links
PubMed source DOI: 10.1074/mcp.M113.032680 PMC Full Text View journal details
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