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PMID: 2434952 Published · ppublish English Journal Article

Abundant expression of homeobox genes in mouse embryonal carcinoma cells correlates with chemically induced differentiation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 5 ·1987-03-00 ·Pages 1304-8

Deschamps J, de Laaf R, Joosen L, Meijlink F, Destrée O

Abstract

Mammalian homeobox-containing genes might play a role in embryonal pattern formation. In favor of this view is the recently reported expression of such genes during mouse embryogenesis [Manley, J. L. & Levine, M. S. (1985) Cell 43, 1-2]. The embryo-derived stem cells and in particular the pluripotent embryonal carcinoma (EC) cell lines are generally considered as a valid model of early mouse development. Homeobox-containing genes were shown to be expressed in differentiating EC cells. We have analyzed the expression of several of these genes in three EC cell lines triggered to differentiate by alternative treatments in the presence or in the absence of retinoic acid. In both types of conditions, C17S1 (clone 1003) and PCC7.S Aza R1 EC cells were induced to differentiate into mainly neurones, and PSA-1 EC cells were induced to differentiate into a large spectrum of tissue derivatives. Induction to high levels of expression of several homeobox-containing genes during differentiation occurs only in the presence of retinoic acid. Nonchemical treatment triggering differentiation does not lead to detectable expression of these genes. Accumulation to high amounts of homeobox-containing gene transcripts in these experiments seems to correlate with retinoic acid-induced EC cell differentiation rather than with EC cell differentiation as such.

MeSH Terms
Animals Cell Differentiation Cell Line Fluorescent Antibody Technique Genes, Homeobox Mice Neurons/cytology Nucleic Acid Hybridization Poly A/genetics RNA/genetics RNA, Messenger Teratoma/genetics,pathology
Chemicals
RNA, Messenger Poly A RNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Deschamps J
de Laaf R
Joosen L
Meijlink F
Destrée O
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31 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.5.1304
Published
1987-03-00
Pages
1304-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC304416
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.84.5.1304 PMC Full Text View journal details
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