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PMID: 2439899 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular analysis of antigen recognition by insulin-specific T-cell hybridomas from B6 wild-type and bm12 mutant mice.

Molecular and cellular biology ·Vol. 7 ·No. 5 ·1987-05-00 ·Pages 1865-72

Sherman DH, Hochman PS, Dick R, Tizard R, Ramachandran KL, Flavell RA, Huber BT

Abstract

Molecular analysis of the heterodimeric T-cell antigen receptor of insulin-specific class II-restricted T-cell hybridomas (THys) derived from C57BL/6 (B6) wild-type and B6.C-H-2bm12 (bm12) mutant mice revealed that such T cells use a diverse V gene repertoire. Analysis of three THys that use related V genes, however, showed a number of novel features. Two THys that share major histocompatibility complex restriction use V alpha genes that are 98.6% homologous. Two THys sharing the same antigen fine specificity use a particular germ line V beta D beta J beta combination. A 21-base-pair deletion in the 5' segment of the J beta gene occurs in one THy, suggesting a novel mechanism for generating diversity in T-cell antigen receptor beta genes. The first amino acid encoded by N sequences at the V-D junction is conserved in a pair of T cells which recognize identical antigenic epitopes. The implications of these findings for the structural mechanisms underlying major histocompatibility complex-restricted antigen-specific T-cell recognition are discussed.

MeSH Terms
Animals Cloning, Molecular DNA/genetics Epitopes Histocompatibility Antigens Class II/immunology Hybridomas/immunology Insulin/immunology Major Histocompatibility Complex Mice Mice, Mutant Strains Peptide Fragments/genetics Receptors, Antigen, T-Cell/genetics,physiology Receptors, Antigen, T-Cell, alpha-beta T-Lymphocytes/immunology
Chemicals
Epitopes Histocompatibility Antigens Class II Insulin Peptide Fragments Receptors, Antigen, T-Cell Receptors, Antigen, T-Cell, alpha-beta DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Sherman D H
Hochman P S
Dick R
Tizard R
Ramachandran K L
Flavell R A
Huber B T
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57 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.5.1865-1872.1987
Published
1987-05-00
Pages
1865-72
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365290
Subset
IM
Grants
NIAID NIH HHS · AI14910 · United States
NIAID NIH HHS · AI23031 · United States
NCI NIH HHS · CA41539 · United States
Databases
GENBANK
M16118, M16119, M16120, M16121, M16122
External Links
PubMed source DOI: 10.1128/mcb.7.5.1865-1872.1987 PMC Full Text View journal details
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