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PMID: 2447584 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Host control of plasmid replication: requirement for the sigma factor sigma 32 in transcription of mini-F replication initiator gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 24 ·1987-12-00 ·Pages 8849-53

Wada C, Imai M, Yura T

Abstract

Replication of F factor or mini-F plasmid is strongly inhibited in the rpoH (htpR) mutants of Escherichia coli deficient in the sigma factor (sigma 32) known to be required for heat shock gene expression. Transcription of the mini-F repE gene encoding a replication initiator protein (E protein) was examined by operon fusion and by direct determination of repE mRNA. The synthesis rate and the level of repE mRNA were found to increase transiently upon temperature upshift (30 degrees C to 42 degrees C) in wild-type cells but to decrease rapidly in the rpoH mutants. Thus sigma 32 appeared to be directly involved in transcription of repE whose product, E protein, in turn activates DNA replication from the mini-F ori2 region. This scheme of host-controlled plasmid replication is further supported by the analysis of transcription in vitro: RNA synthesis can be initiated from the repE promoter by a minor form of RNA polymerase containing sigma 32 but not by the major polymerase containing the normal sigma factor sigma 70. The sigma 32-mediated transcription from the repE promoter is strongly inhibited by the E protein. We conclude that transcription of the mini-F repE gene is mediated by the host transcription factor sigma 32 and is negatively controlled by its own product.

MeSH Terms
Bacterial Proteins/genetics DNA Mutational Analysis DNA Replication DNA-Directed RNA Polymerases/metabolism Escherichia coli/genetics F Factor Genes, Bacterial Promoter Regions, Genetic RNA, Bacterial/genetics RNA, Messenger/genetics Sigma Factor/physiology Transcription Factors/physiology Transcription, Genetic
Chemicals
Bacterial Proteins RNA, Bacterial RNA, Messenger Sigma Factor Transcription Factors DNA-Directed RNA Polymerases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wada C
Institute for Virus Research, Kyoto University, Japan.
Imai M
Yura T
References (34)
34 references, click to expand
  1. RNA polymerase interaction at the promoter--operator region of the tryptophan operon of Escherichia coli and Salmonella typhimurium.
    J Mol Biol. 1978 May 15;121(2):153-77 PMID: 351193
  2. Positive regulatory gene for temperature-controlled proteins in Escherichia coli.
    Biochem Biophys Res Commun. 1981 May 29;100(2):894-900 PMID: 7023474
  3. The construction and characterisation of plasmid vectors suitable for the expression of all DNA phases under the control of the E. coli tryptophan promoter.
    Mol Gen Genet. 1980 Feb;177(3):427-38 PMID: 6246398
  4. Requirement of the Escherichia coli dnaA gene function for ori-2-dependent mini-F plasmid replication.
    J Bacteriol. 1987 Apr;169(4):1724-30 PMID: 3031019
  5. Purification and properties of the mini-F plasmid-encoded E protein needed for autonomous replication control of the plasmid.
    Proc Natl Acad Sci U S A. 1986 Jun;83(12):4109-13 PMID: 3520564
  6. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
    Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 PMID: 6091052
  7. Mechanism of autonomous control of the Escherichia coli F plasmid: different complexes of the initiator/repressor protein are bound to its operator and to an F plasmid replication origin.
    Nucleic Acids Res. 1986 Jul 25;14(14):5693-711 PMID: 3016662
  8. Host participation in plasmid maintenance: dependence upon dnaA of replicons derived from P1 and F.
    Proc Natl Acad Sci U S A. 1986 Jun;83(12):4423-7 PMID: 3520571
  9. Separation of the minimal replication region of the F plasmid into a replication origin segment and a trans-acting segment.
    Mol Gen Genet. 1982;186(3):372-7 PMID: 6750320
  10. Promoter selectivity of Escherichia coli RNA polymerase: alteration by fMet-tRNAfMet.
    Nucleic Acids Res. 1986 Sep 11;14(17):6857-70 PMID: 3532031
  11. Nine unique repeating sequences in a region essential for replication and incompatibility of the mini-F plasmid.
    Gene. 1981 Nov;15(2-3):257-71 PMID: 7028572
  12. Replication and incompatibility of F and plasmids in the IncFI Group.
    Plasmid. 1981 Jan;5(1):100-26 PMID: 7012871
  13. Requirement of the Escherichia coli dnaA gene product for plasmid F maintenance.
    J Bacteriol. 1986 Oct;168(1):440-3 PMID: 3020005
  14. The htpR gene product of E. coli is a sigma factor for heat-shock promoters.
    Cell. 1984 Sep;38(2):383-90 PMID: 6380765
  15. Identification of the minimal essential region for the replication origin of miniF plasmid.
    Mol Gen Genet. 1984;196(2):373-8 PMID: 6593565
  16. Method for the isolation of the replication region of a bacterial replicon: construction of a mini-F'kn plasmid.
    J Bacteriol. 1976 Aug;127(2):982-7 PMID: 783124
  17. Regulated expression of a gene important for replication of plasmid F in E. coli.
    EMBO J. 1984 Feb;3(2):257-62 PMID: 6325163
  18. An Mr 29000 protein is essential for mini-F maintenance in E. coli.
    Gene. 1982 Sep;19(2):173-8 PMID: 6293926
  19. Analysis of mini-F plasmid replication by transposition mutagenesis.
    J Bacteriol. 1981 Sep;147(3):888-99 PMID: 6268613
  20. Nucleotide sequence of the heat shock regulatory gene of E. coli suggests its protein product may be a transcription factor.
    Cell. 1984 Aug;38(1):175-82 PMID: 6088062
  21. Control of F plasmid replication by a host gene: evidence for interaction of the mafA gene product of Escherichia coli with the mini-F incC region.
    J Bacteriol. 1984 Dec;160(3):1130-6 PMID: 6094502
  22. Construction and characterization of plasmid and lambda phage vector systems for study of transcriptional control in Escherichia coli.
    Gene. 1987;57(1):89-99 PMID: 2828183
  23. A two-stage molecular model for control of mini-F replication.
    Plasmid. 1985 Jan;13(1):59-69 PMID: 2986188
  24. Cloning, isolation, and characterization of replication regions of complex plasmid genomes.
    Proc Natl Acad Sci U S A. 1975 Jun;72(6):2242-6 PMID: 1094460
  25. Genetic control of heat-shock protein synthesis and its bearing on growth and thermal resistance in Escherichia coli K-12.
    Proc Natl Acad Sci U S A. 1982 Feb;79(3):860-4 PMID: 7038687
  26. A review of mini-F plasmid maintenance.
    Plasmid. 1985 Jul;14(1):1-16 PMID: 3898165
  27. An amber replication mutant of F plasmid mapped in the minimal replication region.
    Mol Gen Genet. 1983;191(2):231-7 PMID: 6353162
  28. Heat shock regulatory gene (htpR) of Escherichia coli is required for growth at high temperature but is dispensable at low temperature.
    Proc Natl Acad Sci U S A. 1984 Nov;81(21):6803-7 PMID: 6387714
  29. Temperature dependence of sex-factor maintenance in Escherichia coli K-12.
    J Bacteriol. 1972 Jan;109(1):447-9 PMID: 4550674
  30. Evidence for two functional gal promoters in intact Escherichia coli cells.
    J Biol Chem. 1981 Nov 25;256(22):11905-10 PMID: 6271763
  31. The use of operon fusions in studies of the heat-shock response: effects of altered sigma 32 on heat-shock promoter function in Escherichia coli.
    Mol Gen Genet. 1987 Apr;207(1):24-8 PMID: 3299002
  32. Consensus sequence for Escherichia coli heat shock gene promoters.
    Proc Natl Acad Sci U S A. 1985 May;82(9):2679-83 PMID: 3887408
  33. Two functions of the E protein are key elements in the plasmid F replication control system.
    J Bacteriol. 1985 Dec;164(3):1262-70 PMID: 2999077
  34. Inhibition of F plasmid replication in htpR mutants of Escherichia coli deficient in sigma 32 protein.
    Mol Gen Genet. 1986 May;203(2):208-13 PMID: 3526090
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.24.8849
Published
1987-12-00
Pages
8849-53
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299648
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.84.24.8849 PMC Full Text View journal details
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