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PMID: 2452447 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Antibodies that inhibit fusion of human immunodeficiency virus-infected cells bind a 24-amino acid sequence of the viral envelope, gp120.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 9 ·1988-05-00 ·Pages 3198-202

Rusche JR, Javaherian K, McDanal C, Petro J, Lynn DL, Grimaila R, Langlois A, Gallo RC, Arthur LO, Fischinger PJ

Abstract

Antisera to recombinant human immunodeficiency virus (HIV) proteins containing the entire envelope, gp160, or the central portion of the envelope, PB1, can inhibit fusion of virally infected cells in culture. This fusion inhibition is HIV-variant specific--that is, anti-gp160-IIIB inhibits fusion of isolate HTLV-IIIB-infected cells but not of isolate HTLV-IIIRF-infected cells. Both anti-gp160 and anti-PB1 are completely blocked in fusion inhibition activity by the addition of PB1 protein. A 24-amino acid peptide (RP135, amino acids 307-330) completely blocks fusion inhibition activity of both antisera and also blocks the activity of serum from a chimpanzee infected with HTLV-IIIB. Thus, the principal epitope that elicits fusion-inhibiting antibodies is located in the central portion of gp120.

MeSH Terms
Amino Acid Sequence Animals Blood Enzyme-Linked Immunosorbent Assay Epitopes/analysis HIV/immunology HIV Envelope Protein gp120 Pan troglodytes Retroviridae Proteins/immunology Viral Fusion Proteins/antagonists & inhibitors
Chemicals
Epitopes HIV Envelope Protein gp120 Retroviridae Proteins Viral Fusion Proteins
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Rusche J R
Repligen Corporation, Cambridge, MA 02139.
Javaherian K
McDanal C
Petro J
Lynn D L
Grimaila R
Langlois A
Gallo R C
Arthur L O
Fischinger P J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.9.3198
Published
1988-05-00
Pages
3198-202
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC280171
Subset
IM
Grants
NIH HHS · FOD 0758 · United States
PHS HHS · N01-1-62558 · United States
NCI NIH HHS · N01-CO-23910 · United States
Corrections
ErratumIn
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External Links
PubMed source DOI: 10.1073/pnas.85.9.3198 PMC Full Text View journal details
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