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PMID: 2473436 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Substitution of an invariant nucleotide at the base of the highly conserved '530-loop' of 15S rRNA causes suppression of yeast mitochondrial ochre mutations.

Nucleic acids research ·Vol. 17 ·No. 12 ·1989-06-26 ·Pages 4535-9

Shen ZH, Fox TD

Abstract

We have determined the nucleotide sequence alteration in the 15S rRNA gene of a Saccharomyces cerevisiae strain carrying the previously described mitochondrial ochre suppressor, MSUI. The suppressor contains an A residue at position 633 of the yeast mitochondrial sequence, in place of the wild-type G. This position, located in the highly conserved region forming the stem of the '530-loop', corresponds to G517 of the Escherichia coli 16S rRNA and is occupied by G in all other known small rRNA sequences. This finding strongly supports the previous conclusions of others that the 530-loop region plays an important role in enhancing translational accuracy.

MeSH Terms
Base Sequence Genetic Variation Mitochondria/metabolism Molecular Sequence Data Mutation Polymorphism, Genetic RNA/genetics RNA, Fungal/genetics RNA, Mitochondrial RNA, Ribosomal/genetics Saccharomyces cerevisiae/genetics Suppression, Genetic
Chemicals
RNA, Fungal RNA, Mitochondrial RNA, Ribosomal RNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Shen Z H
Section of Genetics and Development, Cornell University, Ithaca, NY 14853-2703.
Fox T D
References (35)
35 references, click to expand
  1. Dideoxy sequencing method using denatured plasmid templates.
    Anal Biochem. 1986 Feb 1;152(2):232-8 PMID: 3516005
  2. A ribosomal ambiguity mutation.
    J Mol Biol. 1969 Jan 14;39(1):95-112 PMID: 4938819
  3. Mutant 16S ribosomal RNA: a codon-specific translational suppressor.
    Proc Natl Acad Sci U S A. 1988 Jun;85(12):4162-5 PMID: 3288986
  4. Genetic and physical analysis of the mitochondrial gene for subunit II of yeast cytochrome c oxidase.
    J Mol Biol. 1979 May 5;130(1):63-82 PMID: 224191
  5. tRNA, suppression, and the code.
    Annu Rev Genet. 1985;19:57-80 PMID: 2417544
  6. A discontinuous small subunit ribosomal RNA in Tetrahymena pyriformis mitochondria.
    J Biol Chem. 1986 Apr 15;261(11):5187-93 PMID: 3082879
  7. A mitochondrial frameshift-suppressor (+1) [corrected] of the yeast S. cerevisiae maps in the mitochondrial 15S rRNA locus.
    Curr Genet. 1987;11(4):295-301 PMID: 3329051
  8. The molecular basis for rRNA-dependent spectinomycin resistance in Nicotiana chloroplasts.
    EMBO J. 1987 Nov;6(11):3233-7 PMID: 16453804
  9. Altered 40 S ribosomal subunits in omnipotent suppressors of yeast.
    J Mol Biol. 1986 Mar 20;188(2):207-14 PMID: 3522920
  10. Ram ribosomes are defective proofreaders.
    Mol Gen Genet. 1983;191(3):378-81 PMID: 6355760
  11. E. coli ribosomes with a C912 to U base change in the 16S rRNA are streptomycin resistant.
    EMBO J. 1986 Dec 20;5(13):3705-8 PMID: 3104030
  12. Suppressor of yeast mitochondrial ochre mutations that maps in or near the 15S ribosomal RNA gene of mtDNA.
    Proc Natl Acad Sci U S A. 1982 Mar;79(5):1583-7 PMID: 6280192
  13. Suppressors in yeast.
    Curr Top Microbiol Immunol. 1974;64(0):1-47 PMID: 4602646
  14. Structure of ribosomal RNA.
    Annu Rev Biochem. 1984;53:119-62 PMID: 6206780
  15. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  16. Leaky +1 and -1 frameshift mutations at the same site in a yeast mitochondrial gene.
    Nature. 1980 Nov 6;288(5786):60-3 PMID: 6253818
  17. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.
    Gene. 1984 Jun;28(3):351-9 PMID: 6235151
  18. Mapping of chloroplast mutations conferring resistance to antibiotics in Chlamydomonas: evidence for a novel site of streptomycin resistance in the small subunit rRNA.
    Mol Gen Genet. 1988 Oct;214(2):192-7 PMID: 3237207
  19. Streptomycin-resistance of Euglena gracilis chloroplasts: identification of a point mutation in the 16S rRNA gene in an invariant position.
    Nucleic Acids Res. 1985 Jun 25;13(12):4299-310 PMID: 3925438
  20. Mitochondrial and nuclear mutations that affect the biogenesis of the mitochondrial ribosomes of yeast. I. Genetics.
    Mol Gen Genet. 1979;177(1):39-46 PMID: 395414
  21. Mutational study of the rRNA in yeast mitochondria: functional importance of T1696 in the large rRNA gene.
    Nucleic Acids Res. 1988 Oct 11;16(19):9299-306 PMID: 3050898
  22. Sequence of the intron and flanking exons of the mitochondrial 21S rRNA gene of yeast strains having different alleles at the omega and rib-1 loci.
    Cell. 1980 May;20(1):185-97 PMID: 6156002
  23. High resolution localization of the tRNA anticodon interaction site on the Escherichia coli 30 S ribosomal subunit.
    J Biol Chem. 1984 Aug 25;259(16):10493-8 PMID: 6381487
  24. Mitochondrial DNA of chloramphenicol-resistant mouse cells contains a single nucleotide change in the region encoding the 3' end of the large ribosomal RNA.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3789-93 PMID: 6943583
  25. A mutation in the 530 loop of Escherichia coli 16S ribosomal RNA causes resistance to streptomycin.
    Nucleic Acids Res. 1988 Oct 25;16(20):9631-9 PMID: 3054810
  26. Identification of the paromomycin-resistance mutation in the 15 S rRNA gene of yeast mitochondria.
    J Biol Chem. 1982 May 25;257(10):5921-8 PMID: 6279619
  27. Leakiness of termination codons in mitochondrial mutants of the yeast Saccharomyces cerevisiae.
    Mol Gen Genet. 1984;198(2):62-8 PMID: 6394966
  28. Interaction of antibiotics with functional sites in 16S ribosomal RNA.
    Nature. 1987 Jun 4-10;327(6121):389-94 PMID: 2953976
  29. Transfer RNA shields specific nucleotides in 16S ribosomal RNA from attack by chemical probes.
    Cell. 1986 Dec 26;47(6):985-94 PMID: 2430725
  30. DNA hybridization electron microscopy: ribosomal RNA nucleotides 1392-1407 are exposed in the cleft of the small subunit.
    Proc Natl Acad Sci U S A. 1986 Jan;83(2):275-9 PMID: 3510432
  31. Site-specific AT-cluster insertions in the mitochondrial 15S rRNA gene of the yeast S. cerevisiae.
    Nucleic Acids Res. 1988 Sep 12;16(17):8665-74 PMID: 2843821
  32. Unexpectedly long 14S ribosomal RNA gene in Tetrahymena mitochondria.
    Curr Genet. 1987;11(6-7):529-36 PMID: 2453300
  33. A cold-sensitive cytoplasmic mutation of Saccharomyces cerevisiae affecting assembly of the mitochondrial 50S ribosomal subunit.
    Mol Gen Genet. 1978 May 3;161(2):143-51 PMID: 353505
  34. Involvement of ribosomal protein L7/L12 in control of translational accuracy.
    Proc Natl Acad Sci U S A. 1985 Feb;82(3):717-21 PMID: 3883346
  35. Localization of the binding site for protein S4 on 16 S ribosomal RNA by chemical and enzymatic probing and primer extension.
    J Mol Biol. 1986 Nov 5;192(1):101-10 PMID: 3820298
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.12.4535
Published
1989-06-26
Pages
4535-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318012
Subset
IM
Grants
NIGMS NIH HHS · GM29362 · United States
Databases
GENBANK
14966, X14966
External Links
PubMed source DOI: 10.1093/nar/17.12.4535 PMC Full Text View journal details
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