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PMID: 6355760 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Ram ribosomes are defective proofreaders.

Molecular & general genetics : MGG ·Vol. 191 ·No. 3 ·1983-00-00 ·Pages 378-81

Andersson DI, Kurland CG

Abstract

We have studied the kinetics of poly(U) translation by three ribosomal ambiguity (Ram) mutants in an in vitro system with performance characteristics similar to those expressed in vivo. The leucine missense frequency supported by Ram ribosomes with tRNALeu2 increases between six and twelve-fold over that of wild-type ribosomes, while the corresponding increase with tRNALeu4 was between four and eight-fold, depending on the rpsD allele. We have used a steady-state assay for proofreading to identify the kinetic lesion responsible for the Ram phenotype. We were unable to detect any difference between Ram and wild-type ribosomes with respect to the initial kinetics of amino-acyl tRNA selection. All of the increased error rates could be associated with a decreased capacity of these Ram ribosomes to discard non-cognate aminoacyl-tRNA by proof reading.

MeSH Terms
Escherichia coli/genetics Kinetics Leucine Mutation Poly U/metabolism Protein Biosynthesis RNA, Transfer/genetics Ribosomal Proteins/genetics Ribosomes/physiology,ultrastructure Structure-Activity Relationship
Chemicals
Ribosomal Proteins Poly U RNA, Transfer Leucine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Andersson D I
Kurland C G
References (19)
19 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00425749
Published
1983-00-00
Pages
378-81
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00425749
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