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PMID: 3883346 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Involvement of ribosomal protein L7/L12 in control of translational accuracy.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 3 ·1985-02-00 ·Pages 717-21

Kirsebom LA, Isaksson LA

Abstract

The effects of two mutations, which map at the rplL locus and both give a changed 50S ribosomal protein L7/L12, were studied. Both mutations are associated with an increased misreading of all three nonsense codons in vivo and ribosomes from the mutants give an increased misreading of the phenylalanine codon UUU by tRNALeu in vitro. The rplL-associated misreading in vitro is not limited to a particular type of mRNA or tRNA. Results from a translational proofreading assay, using mutant ribosomes, suggest that protein L7/L12 is involved in the control of translational accuracy by contributing to the efficiency of a translational proofreading step(s).

MeSH Terms
Bacterial Proteins/biosynthesis,physiology Base Sequence Codon Escherichia coli/genetics Genotype Kinetics Leucine/metabolism Mutation Poly U/metabolism Protein Biosynthesis Ribosomal Proteins/physiology
Chemicals
Bacterial Proteins Codon Ribosomal Proteins Poly U ribosomal protein L7-L12 Leucine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kirsebom L A
Isaksson L A
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37 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.3.717
Published
1985-02-00
Pages
717-21
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397117
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.82.3.717 PMC Full Text View journal details
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