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PMID: 2477241 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

vav, a novel human oncogene derived from a locus ubiquitously expressed in hematopoietic cells.

The EMBO journal ·Vol. 8 ·No. 8 ·1989-08-00 ·Pages 2283-90

Katzav S, Martin-Zanca D, Barbacid M

Abstract

A novel human oncogene, designated vav, was generated by a genetic rearrangement during gene transfer assays. The vav oncogene directs the synthesis of a 3.0 kb mRNA from which we isolated a 2.8 kb-long complementary DNA copy. Nucleotide sequence analysis of this vav oncogene cDNA clone revealed that its 5' 167 bp were derived from pSV2neo DNA cotransfected as a selectable marker during gene transfer. The remaining 2597 bp were unrelated to genes included in current data banks, indicating that the vav oncogene is likely to be derived from a novel human locus. The vav oncogene cDNA clone encompasses a 2391 bp long open reading frame (ORF) capable of directing the synthesis of a 797 amino acid long polypeptide. The predicted vav oncogene protein sequence exhibits several motifs reminiscent of transcriptional factors. They include a highly acidic amino-terminal region separated from two putative nuclear localization signals by a proline-rich sequence, presumably a hinge region. In addition, we identified two zinc-finger-like domains, one of which conforms to the canonical pattern Cys-X2-Cys-X13-Cys-X2-Cys previously found to confer trans-activating activity to the adenovirus E1A protein. Transcription of its normal allele, the vav proto-oncogene, has been exclusively observed in cells of hematopoietic origin, including those of erythroid, lymphoid and myeloid lineages. These findings raise the possibility that this novel locus might play an important role in hematopoiesis.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Blood Cells/metabolism Blotting, Northern Blotting, Southern Cell Line DNA/genetics,isolation & purification Esophageal Neoplasms/genetics Exons Gene Expression Regulation Gene Rearrangement Humans Introns Mice Molecular Sequence Data Oncogene Proteins/genetics Plasmids Poly A/genetics Proto-Oncogene Mas Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins c-vav Proto-Oncogenes RNA/genetics RNA, Messenger Sequence Homology, Nucleic Acid Transformation, Genetic
Chemicals
MAS1 protein, human Oncogene Proteins Proto-Oncogene Mas Proto-Oncogene Proteins Proto-Oncogene Proteins c-vav RNA, Messenger VAV1 protein, human Vav1 protein, mouse Poly A RNA DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Katzav S
Department of Oncology Section, Frederick Cancer Research Facility, MD 21701.
Martin-Zanca D
Barbacid M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-08-00
Pages
2283-90
Language
English
Region
England
NLM ID
8208664
PMCID
PMC401160
Subset
IM
Grants
NCI NIH HHS · N01-CO-74101 · United States
External Links
PubMed source PMC Full Text View journal details
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