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PMID: 2513479 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Functional serum response elements upstream of the growth factor-inducible gene zif268.

Molecular and cellular biology ·Vol. 9 ·No. 11 ·1989-11-00 ·Pages 4889-95

Christy B, Nathans D

Abstract

The zif268 gene, which encodes a protein with three typical zinc finger sequences, is induced in mouse 3T3 cells by serum, phorbol 12-myristate 13-acetate platelet-derived growth factor, and fibroblast growth factor. The induction is coordinate with that of c-fos. The 5'-flanking region of zif268 contains sequences that resemble known regulatory elements, including four CC(A or T)6GG sequences similar to the core serum response elements (SREs) found upstream of c-fos and actin genes. To determine whether the zif268 SRE-like elements mediate induction, CAT (chloramphenicol acetyltransferase) plasmids with different lengths of zif268 upstream sequences were tested for inducibility in 3T3 cells by serum, platelet-derived growth factor, or phorbol 12-myristate 13-acetate. In addition, double-stranded oligonucleotides corresponding to each of the four zif268 putative SREs were tested individually for responsiveness when placed upstream of a thymidine kinase gene promoter. Each of the four SREs conferred inducibility by the agents tested, and multiple SREs resulted in greater inducibility than did a single element. Each of the zif268 SREs also competed with the c-fos SRE for binding by serum response factor present in HeLa cell nuclear extract. We conclude that the zif268 SRE-like sequences are functional and probably account for the coordinate induction of zif268 and c-fos.

MeSH Terms
Animals Base Sequence Binding, Competitive Blood Cell Line Chloramphenicol O-Acetyltransferase/metabolism DNA Mutational Analysis DNA-Binding Proteins/genetics,metabolism Fibroblast Growth Factors/pharmacology Gene Expression Regulation Genes Metalloproteins/genetics Mice Molecular Sequence Data Plasmids Platelet-Derived Growth Factor/pharmacology Regulatory Sequences, Nucleic Acid/genetics Tetradecanoylphorbol Acetate/pharmacology Thymidine Kinase/genetics Transfection Zinc
Chemicals
DNA-Binding Proteins Metalloproteins Platelet-Derived Growth Factor Fibroblast Growth Factors Chloramphenicol O-Acetyltransferase Thymidine Kinase Zinc Tetradecanoylphorbol Acetate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Christy B
Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Nathans D
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49 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.11.4889-4895.1989
Published
1989-11-00
Pages
4889-95
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363639
Subset
IM
Grants
NCI NIH HHS · 5 PO1 CA16519 · United States
External Links
PubMed source DOI: 10.1128/mcb.9.11.4889-4895.1989 PMC Full Text View journal details
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