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PMID: 25159149 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Contractile ring stability in S. pombe depends on F-BAR protein Cdc15p and Bgs1p transport from the Golgi complex.

Cell reports ·Vol. 8 ·No. 5 ·2014-09-11 ·Pages 1533-44

Arasada R, Pollard TD

Abstract

Cdc15p is known to contribute to cytokinesis in fission yeast; however, the protein is not required to assemble the contractile ring of actin and myosin, but it helps to anchor the ring to the plasma membrane. Cdc15p has a lipid-binding F-BAR domain, suggesting that it provides a physical link between the plasma membrane and contractile ring proteins. However, we find that a more important function of Cdc15p during cytokinesis is to help deliver a transmembrane enzyme, Bgs1p (also called Cps1p), from the Golgi apparatus to the plasma membrane, where it appears to anchor the contractile ring. Bgs1p synthesizes the cell wall in the cleavage furrow, but its enzyme activity is not required to anchor the contractile ring. We estimate that ∼ 2,000 Bgs1p molecules are required to anchor the ring. Without Bgs1p anchors, contractile rings slide along the plasma membrane, a phenomenon that depends on an unconventional type II myosin called Myp2p.

MeSH Terms
Cell Cycle Proteins/chemistry,genetics,metabolism Cell Membrane/metabolism Cytokinesis GTP-Binding Proteins/chemistry,genetics,metabolism Glucosyltransferases/genetics,metabolism Golgi Apparatus/metabolism Myosin Heavy Chains/metabolism Protein Binding Protein Structure, Tertiary Protein Transport Schizosaccharomyces/genetics,metabolism,physiology Schizosaccharomyces pombe Proteins/metabolism
Chemicals
CDC15 protein Cell Cycle Proteins Myp2 protein, S pombe Schizosaccharomyces pombe Proteins Glucosyltransferases 1,3-beta-glucan synthase GTP-Binding Proteins Myosin Heavy Chains
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Arasada Rajesh
Department of Molecular Cellular and Developmental Biology, Yale University, P.O. Box 208103, New Haven, CT 06520-8103 USA.
Pollard Thomas D
Department of Molecular Cellular and Developmental Biology, Yale University, P.O. Box 208103, New Haven, CT 06520-8103 USA; Department of Molecular Biophysics and Biochemistry, Yale University, P.O. Box 208103, New Haven, CT 06520-8103 USA; Department of Cell Biology, Yale University, P.O. Box 208103, New Haven, CT 06520-8103 USA. Electronic address: [email protected].
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Article Info
Journal
Cell reports
Abbr.
Cell Rep
ISSN
2211-1247
DOI
10.1016/j.celrep.2014.07.048
Email
[email protected]
Published
2014-09-11
Epub
2014-00-21
Pages
1533-44
Language
English
Region
United States
NLM ID
101573691
PMCID
PMC4163078
Subset
IM
Grants
NIGMS NIH HHS · R01 GM026132 · United States
NIGMS NIH HHS · R01 GM026338 · United States
NIGMS NIH HHS · GM-026132 · United States
NIGMS NIH HHS · GM-026338 · United States
External Links
PubMed source DOI: 10.1016/j.celrep.2014.07.048 PMC Full Text View journal details
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