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PMID: 2524382 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Regulation of tissue-specific alternative splicing: exon-specific cis-elements govern the splicing of leukocyte common antigen pre-mRNA.

The EMBO journal ·Vol. 8 ·No. 3 ·1989-03-00 ·Pages 787-96

Streuli M, Saito H

Abstract

Tissue-specific alternative splicing is an important mechanism for controlling gene expression. Exons 4, 5 and 6 of the human leukocyte common antigen (LCA) gene are included in B cell mRNA but excluded from thymocyte mRNA by differential splicing. In order to study this tissue-specific alternative splicing, we constructed mini-genes that contain only a few of the LCA exons and the SV40 promoter. Mouse B cells and thymocytes were transfected with these mini-gene constructs and the structures of mRNAs were determined by primer extension analysis. The results show that the same primary transcript is spliced alternatively in B cells and thymocytes. This finding suggests that there is a tissue-specific trans-acting factor that regulates the alternative splicing of LCA pre-mRNA. By making various deletion mutants, cis-elements necessary for tissue-specific splicing were confined within the alternatively spliced exons and their immediate flanking intron sequences. Furthermore, linker scanning analysis shows that there are at least three distinct cis-elements within the LCA exon 4 sequence that are required for tissue-specific alternative splicing. Possible mechanisms of LCA alternative splicing are discussed.

MeSH Terms
Antigens, Differentiation/genetics B-Lymphocytes/immunology,metabolism Base Sequence Exons Gene Expression Regulation Histocompatibility Antigens/genetics Humans Leukocyte Common Antigens Molecular Sequence Data RNA Precursors/genetics RNA Splicing T-Lymphocytes/immunology,metabolism Tissue Distribution Transcription Factors/genetics,metabolism
Chemicals
Antigens, Differentiation Histocompatibility Antigens RNA Precursors Transcription Factors Leukocyte Common Antigens
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Streuli M
Department of Pathology, Harvard Medical School, Boston, MA 02115.
Saito H
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-03-00
Pages
787-96
Language
English
Region
England
NLM ID
8208664
PMCID
PMC400875
Subset
IM
Grants
NIAID NIH HHS · AI-26598 · United States
External Links
PubMed source PMC Full Text View journal details
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