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PMID: 2532358 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Costimulatory signal provided by a B-lymphoblastoid cell line and its Ia-negative variant.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 24 ·1989-12-00 ·Pages 10069-73

Reiser H, Benacerraf B

Abstract

We have analyzed the requirements of highly purified, resting murine CD4+ T lymphocytes for activation mediated by the lectin Con A and by monoclonal antibodies against the CD3 and Thy-1 molecules. Our results indicate that both the Ia-positive B-lymphoblastoid cell line M12 and its Ia-negative variant M12.C3 can provide the costimulatory activity necessary for these activation pathways. The costimulatory function is preserved upon fixation with paraformaldehyde, indicating that the costimulatory molecule(s) is (are) constitutively expressed on the cell surface. Our experiments also point to interesting differences between the M12 cell line and syngeneic Ia-positive antigen-presenting cells in generating a syngeneic mixed lymphocyte reaction. Finally, we show that the CD4+ T cell-M12.C3 cell interaction can be used to screen for interesting monoclonal antibodies that affect cell function.

MeSH Terms
Animals Antibodies, Monoclonal Antigen-Antibody Complex B-Lymphocytes/immunology CD4 Antigens/immunology Cell Communication Cell Line Cells, Cultured Cricetinae Genetic Variation Histocompatibility Antigens Class II/genetics Lymphocyte Activation Lymphocyte Culture Test, Mixed Mice Phenotype Spleen/immunology T-Lymphocytes/immunology
Chemicals
Antibodies, Monoclonal Antigen-Antibody Complex CD4 Antigens Histocompatibility Antigens Class II
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Reiser H
Department of Pathology, Harvard Medical School, Dana-Farber Cancer Institute, Boston, MA.
Benacerraf B
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46 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.24.10069
Published
1989-12-00
Pages
10069-73
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC298645
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.86.24.10069 PMC Full Text View journal details
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