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PMID: 2535727 Published · ppublish English Journal Article

Properties of a murine retroviral recombinant of avian acute leukemia virus E26: a murine fibroblast assay for v-ets function.

Journal of virology ·Vol. 63 ·No. 1 ·1989-01-00 ·Pages 205-15

Yuan CC, Kan N, Dunn KJ, Papas TS, Blair DG

Abstract

A replication-defective murine retroviral construct, termed pME26, was generated by inserting avian gag-myb-ets sequences derived from the cloned avian acute leukemia virus E26 into an Abelson murine leukemia virus-derived retroviral vector. ME26 virus can be rescued efficiently from transfected NIH 3T3 cells by replicating murine leukemia viruses. Either pME26-transfected nonproducers or ME26 virus-infected NIH 3T3 cells expressed a 135-kilodalton fusion protein (p135) which was detectable by immunoprecipitation with antiserum directed against avian leukemia virus p27gag, myb or ets oncogene protein, or murine leukemia virus p15gag and was principally localized in the nucleus. NIH 3T3 cells infected with ME26 exhibited morphological alterations and increased proliferation in reduced serum and formed small colonies in agar suspension. Discrete foci could be readily recognized in cells maintained in a defined medium containing 0.03 to 0.1% calf serum. In newborn NFS/N mice, ME26 induced a significantly higher mortality and incidence of erythroid and myeloid leukemias. Analysis of a series of mutants affecting the expression of various portions of p135 indicated that the v-ets gene acts to mitogenically stimulate the proliferation of NIH 3T3 fibroblasts and reduces or abolishes their serum dependence. These properties provide an assay system to study functions of the ets gene family.

MeSH Terms
Animals Avian Leukosis Virus/genetics Blotting, Northern Blotting, Southern Cell Line DNA/analysis Fibroblasts Genes, Viral Leukemia, Experimental/microbiology Liver/analysis Lymphoma/microbiology Mice Mutation Oncogenes Plasmids Precipitin Tests Proviruses/genetics RNA, Viral/analysis Retroviridae Proteins/genetics,physiology Retroviridae Proteins, Oncogenic Spleen/analysis Transfection
Chemicals
RNA, Viral Retroviridae Proteins Retroviridae Proteins, Oncogenic oncogene proteins v-ets DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Yuan C C
Laboratory of Molecular Oncology, National Cancer Institute, Frederick, Maryland 21701-1013.
Kan N
Dunn K J
Papas T S
Blair D G
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.63.1.205-215.1989
Published
1989-01-00
Pages
205-15
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC247674
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.63.1.205-215.1989 PMC Full Text View journal details
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