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PMID: 2538653 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The Epstein-Barr virus (EBV) BZLF1 immediate-early gene product differentially affects latent versus productive EBV promoters.

Journal of virology ·Vol. 63 ·No. 4 ·1989-04-00 ·Pages 1729-36

Kenney S, Kamine J, Holley-Guthrie E, Lin JC, Mar EC, Pagano J

Abstract

The Epstein-Barr virus (EBV) BZLF1 gene product is thought to mediate the disruption of latent EBV infection. We have examined the regulatory effects of BZLF1 by studying its transactivating effects on seven different EBV promoters. We find that whereas the BZLF1 gene product increases the activity of the two early promoters, BMLF1 and BMRF1, it decreases the activity of three latent promoters (the BamHI-C and BamHI-W Epstein-Barr nuclear antigen promoters and the latent membrane protein promoter). The BZLF1-induced changes in promoter-directed chloramphenicol acetyltransferase activity occur in EBV-negative as well as EBV-positive cell lines and are accompanied by a similar change in chloramphenicol acetyltransferase mRNA. Deletion analysis of the BamHI Z fragment indicates that in a portion of the amino-terminal half of the BZLF1 gene product (amino acids 24 to 86) is not essential for positive transactivating effects but is required for down-regulating effects. Thus, different domains of the same EBV immediate-early gene product can either increase the function of EBV promoters active in productive infection or decrease the function of key promoters active in latent infection.

MeSH Terms
Cell Line DNA Mutational Analysis Gene Expression Regulation Genes, Viral Herpesvirus 4, Human/genetics Humans Promoter Regions, Genetic Protein Biosynthesis RNA, Viral/genetics Transcription Factors/genetics Transcription, Genetic Virus Replication
Chemicals
RNA, Viral Transcription Factors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Kenney S
Department of Medicine, University of North Carolina, Chapel Hill 27599.
Kamine J
Holley-Guthrie E
Lin J C
Mar E C
Pagano J
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.63.4.1729-1736.1989
Published
1989-04-00
Pages
1729-36
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC248433
Subset
IM
Grants
NCI NIH HHS · 1 K08 CA 01 229-01 · United States
NIAID NIH HHS · 1 VOI AI23634-01 · United States
PHS HHS · A10751 · United States
External Links
PubMed source DOI: 10.1128/JVI.63.4.1729-1736.1989 PMC Full Text View journal details
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