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PMID: 2542618 Published · ppublish English Journal Article

The spliced BZLF1 gene of Epstein-Barr virus (EBV) transactivates an early EBV promoter and induces the virus productive cycle.

Journal of virology ·Vol. 63 ·No. 7 ·1989-07-00 ·Pages 3109-16

Rooney CM, Rowe DT, Ragot T, Farrell PJ

Abstract

A spliced cDNA spanning the Epstein-Barr virus BZLF1 gene expresses the BZLF1 protein and is active in inducing the virus productive cycle. A deletion mutant which lacks the N-terminal half of the protein is inactive. Cotransfection experiments in EBV-negative B-lymphocyte cell lines demonstrated that the BZLF1 gene activates the promoter for the BSLF2 + BMLF1 gene in the absence of any other EBV gene product. These results confirmed that the spliced BZLF1 gene is the transactivating gene structure in BamHI-Z.

MeSH Terms
Amino Acid Sequence Base Sequence Cell Line DNA, Recombinant Gene Expression Regulation Genes Genes, Viral Herpesvirus 4, Human/genetics,physiology Humans Molecular Sequence Data Oncogene Proteins, Viral/genetics Plasmids Promoter Regions, Genetic RNA, Messenger/genetics Restriction Mapping Transcription, Genetic Transfection Virus Replication
Chemicals
DNA, Recombinant Oncogene Proteins, Viral RNA, Messenger
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Rooney C M
Ludwig Institute for Cancer Research, St. Mary's Hospital Medical School, London, England.
Rowe D T
Ragot T
Farrell P J
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.63.7.3109-3116.1989
Published
1989-07-00
Pages
3109-16
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC250868
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.63.7.3109-3116.1989 PMC Full Text View journal details
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