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PMID: 2549543 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Genome organization of the anaerobic pathogen Clostridium perfringens.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 17 ·1989-09-00 ·Pages 6676-80

Canard B, Cole ST

Abstract

A physical map of the genome of Clostridium perfringens, an important human pathogen, has been established by pulsed-field gel electrophoresis. Recognition sites for six rare-cutting endonucleases were situated on a single circular chromosome of approximately 3.6 million base pairs thus defining 50 arbitrary genetic intervals of between 10 and 250 kilobase pairs. This considerably facilitated the chromosomal localization of some 24 genes and loci for which probes were available and allowed the construction of the genome map of a clostridial species.

MeSH Terms
Chromosome Mapping Chromosomes, Bacterial Clostridium perfringens/genetics DNA Topoisomerases, Type II/genetics DNA, Bacterial/genetics Electrophoresis/methods Genes Genes, Bacterial RNA, Ribosomal/genetics RNA, Transfer/genetics Restriction Mapping
Chemicals
DNA, Bacterial RNA, Ribosomal RNA, Transfer DNA Topoisomerases, Type II
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Canard B
Laboratoire de Génétique Moléculaire Bactérienne, Institut Pasteur, Paris, France.
Cole S T
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.17.6676
Published
1989-09-00
Pages
6676-80
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC297908
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.86.17.6676 PMC Full Text View journal details
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