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PMID: 25514766 Published · ppublish English Journal Article Review

Detection and manipulation of phosphoinositides.

Biochimica et biophysica acta ·Vol. 1851 ·No. 6 ·2015-06-00 ·Pages 736-45

Idevall-Hagren O, De Camilli P

Abstract

Phosphoinositides (PIs) are minor components of cell membranes, but play key roles in cell function. Recent refinements in techniques for their detection, together with imaging methods to study their distribution and changes, have greatly facilitated the study of these lipids. Such methods have been complemented by the parallel development of techniques for the acute manipulation of their levels, which in turn allow bypassing the long-term adaptive changes implicit in genetic perturbations. Collectively, these advancements have helped elucidate the role of PIs in physiology and the impact of the dysfunction of their metabolism in disease. Combining methods for detection and manipulation enables the identification of specific roles played by each of the PIs and may eventually lead to the complete deconstruction of the PI signaling network. Here, we review current techniques used for the study and manipulation of cellular PIs and also discuss advantages and disadvantages associated with the various methods. This article is part of a Special Issue entitled Phosphoinositides.

Keywords
Cryptochrome Lipid binding domain Optical manipulation Optogenetics Phosphatidylinositol Rapamycin
MeSH Terms
Antibodies/pharmacology Cell Membrane/chemistry,metabolism Chromatography, High Pressure Liquid Chromatography, Thin Layer Enzyme Inhibitors/pharmacology Fluorescence Resonance Energy Transfer Gene Targeting/methods Humans Microscopy Optogenetics Phosphatidylinositols/antagonists & inhibitors,isolation & purification,metabolism Phosphotransferases (Alcohol Group Acceptor)/antagonists & inhibitors,genetics,metabolism Protein Structure, Tertiary Signal Transduction
Chemicals
Antibodies Enzyme Inhibitors Phosphatidylinositols Phosphotransferases (Alcohol Group Acceptor)
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Idevall-Hagren Olof
Department of Medical Cell Biology, Uppsala University, BMC Box 571, 75123 Uppsala, Sweden. Electronic address: [email protected].
De Camilli Pietro
Department of Cell Biology, Howard Hughes Medical Institute and Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, CT 06510, USA. Electronic address: [email protected].
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Article Info
Journal
Biochimica et biophysica acta
Abbr.
Biochim Biophys Acta
ISSN
0006-3002
DOI
10.1016/j.bbalip.2014.12.008
Email
[email protected]
[email protected]
Published
2015-06-00
Epub
2014-00-13
Pages
736-45
Language
English
Region
Netherlands
NLM ID
0217513
PMCID
PMC4863076
Subset
IM
Grants
NIDA NIH HHS · P30 DA018343 · United States
NIDDK NIH HHS · R01 DK082700 · United States
NINDS NIH HHS · R37 NS036251 · United States
External Links
PubMed source DOI: 10.1016/j.bbalip.2014.12.008 PMC Full Text
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