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PMID: 2555252 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Ty1 transposition in Saccharomyces cerevisiae is nonrandom.

Genetics ·Vol. 123 ·No. 2 ·1989-10-00 ·Pages 269-79

Natsoulis G, Thomas W, Roghmann MC, Winston F, Boeke JD

Abstract

A large collection of Ty1 insertions in the URA3 and LYS2 loci was generated using a GAL1-Ty1 fusion to augment the transposition frequency. The sites of insertion of most of these Ty elements were sequenced. There appears to be a gradient of frequency of insertion from the 5' end (highest frequency) to the 3' end (lowest frequency) of both loci. In addition we observed hotspots for transposition. Twelve of the 82 Ty1 insertions in the URA3 locus were inserted in exactly the same site. Hotspots were also observed in the LYS2 locus. All hotspots were in the transcribed part of the genes. Alignment of the sites of insertion and of the neighboring sequences only reveals very weak sequence similarities.

MeSH Terms
Base Composition Base Sequence Cloning, Molecular DNA Transposable Elements DNA, Fungal/genetics Exons Genes, Fungal Mutation Phenotype Plasmids Restriction Mapping Saccharomyces cerevisiae/genetics Sequence Homology, Nucleic Acid Transcription, Genetic
Chemicals
DNA Transposable Elements DNA, Fungal
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Natsoulis G
Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Thomas W
Roghmann M C
Winston F
Boeke J D
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/123.2.269
Published
1989-10-00
Pages
269-79
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1203799
Subset
IM
Grants
NIGMS NIH HHS · GM-32967 · United States
NIGMS NIH HHS · GM-36481 · United States
External Links
PubMed source DOI: 10.1093/genetics/123.2.269 PMC Full Text View journal details
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