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PMID: 2854194 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Ty3, a yeast retrotransposon associated with tRNA genes, has homology to animal retroviruses.

Molecular and cellular biology ·Vol. 8 ·No. 12 ·1988-12-00 ·Pages 5245-56

Hansen LJ, Chalker DL, Sandmeyer SB

Abstract

Ty3, a retrotransposon of Saccharomyces cerevisiae, is found within 20 base pairs (bp) of the 5' ends of different tRNA genes. Determination of the complete nucleotide sequence of one Ty3 retrotransposon (Ty3-2) shows that the element is composed of an internal domain 4,748 bp long flanked by long terminal repeats of the 340-bp sigma element. Three open reading frames (ORFs) longer than 100 codons are present in the sense strand. The first ORF, TYA3, encodes a protein with a motif found in the nucleic acid-binding protein of retroviruses. The second ORF, TYB3, has homology to retroviral pol genes. The deduced amino acid sequence of the reverse transcriptase domain shows the greatest similarity to Drosophila retrotransposon 17.6, with 43% identical residues. The inferred order of functional domains within TYB3--protease, reverse transcriptase, and endonuclease--resembles the order in Drosophila element 17.6 and in animal retroviruses but is different from that found in yeast elements Ty1 and Ty2. A second Ty3 element (Ty3-1) from a standard laboratory strain was overexpressed and shown to transpose.

MeSH Terms
Amino Acid Sequence Base Sequence DNA Transposable Elements Genes Genes, Fungal Genes, Viral Molecular Sequence Data RNA, Transfer/genetics Restriction Mapping Retroviridae/genetics Retroviridae Proteins/genetics Saccharomyces cerevisiae/genetics Sequence Homology, Nucleic Acid
Chemicals
DNA Transposable Elements Retroviridae Proteins RNA, Transfer
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hansen L J
Department of Microbiology and Molecular Genetics, College of Medicine, University of California, Irvine 92717.
Chalker D L
Sandmeyer S B
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.12.5245-5256.1988
Published
1988-12-00
Pages
5245-56
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365627
Subset
IM
Grants
NIGMS NIH HHS · GM07134 · United States
NIGMS NIH HHS · GM33281 · United States
Databases
GENBANK
M23367
External Links
PubMed source DOI: 10.1128/mcb.8.12.5245-5256.1988 PMC Full Text View journal details
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