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PMID: 2581933 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Control of Klebsiella pneumoniae nif mRNA synthesis.

Journal of bacteriology ·Vol. 162 ·No. 3 ·1985-06-00 ·Pages 1186-90

Collins JJ, Brill WJ

Abstract

Four probes, each specific for a single nif transcript, were used for an analysis of the regulation of nif mRNA synthesis. Transcription of the nifLA operon was repressed by NH4+ but not by amino acids, O2, or temperatures above 37 degrees C. The nifA gene product was required for the activation of transcription of the other nif operons but not nifLA. Synthesis of the other nif transcripts was rapidly turned off by the addition of O2, NH4+, serine, or glutamine. These regulatory effects required the nifL product. However, the nifL product was not required for the cessation of synthesis of these transcripts at elevated temperatures.

MeSH Terms
Gene Expression Regulation Klebsiella pneumoniae/genetics,metabolism Nitrogen Fixation Quaternary Ammonium Compounds/pharmacology RNA, Bacterial/biosynthesis RNA, Messenger/biosynthesis Temperature Transcription, Genetic
Chemicals
Quaternary Ammonium Compounds RNA, Bacterial RNA, Messenger
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Collins J J
Brill W J
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34 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.162.3.1186-1190.1985
Published
1985-06-00
Pages
1186-90
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215902
Subset
IM
Grants
NIGMS NIH HHS · GM 22130 · United States
External Links
PubMed source DOI: 10.1128/jb.162.3.1186-1190.1985 PMC Full Text View journal details
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