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PMID: 26411296 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Review

Genetic code flexibility in microorganisms: novel mechanisms and impact on physiology.

Nature reviews. Microbiology ·Vol. 13 ·No. 11 ·2015-11-00 ·Pages 707-721

Ling J, O'Donoghue P, Söll D

Abstract

The genetic code, initially thought to be universal and immutable, is now known to contain many variations, including biased codon usage, codon reassignment, ambiguous decoding and recoding. As a result of recent advances in the areas of genome sequencing, biochemistry, bioinformatics and structural biology, our understanding of genetic code flexibility has advanced substantially in the past decade. In this Review, we highlight the prevalence, evolution and mechanistic basis of genetic code variations in microorganisms, and we discuss how this flexibility of the genetic code affects microbial physiology.

MeSH Terms
Archaea/genetics Bacteria/genetics Codon Evolution, Molecular Genetic Code
Chemicals
Codon
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ling Jiqiang
Department of Microbiology and Molecular Genetics, University of Texas Health Science Center, Houston, Texas 77030, USA.
O'Donoghue Patrick
Department of Biochemistry, The University of Western Ontario, London, Ontario N6A 5C1, Canada. | Department of Chemistry, The University of Western Ontario, London, Ontario N6A 5C1, Canada.
Söll Dieter
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520-8114, USA. | Department of Chemistry, Yale University, New Haven, Connecticut 06520-8114, USA.
References (142)
142 references, click to expand
  1. Translational recoding in archaea.
    Extremophiles. 2012 Nov;16(6):793-803 PMID: 23015064
  2. Transfer ribonucleic acid-mediated suppression of termination codons in Escherichia coli.
    Microbiol Rev. 1988 Sep;52(3):354-74 PMID: 3054467
  3. In vivo requirement of selenophosphate for selenoprotein synthesis in archaea.
    Mol Microbiol. 2010 Jan;75(1):149-60 PMID: 19919669
  4. Causes and effects of N-terminal codon bias in bacterial genes.
    Science. 2013 Oct 25;342(6157):475-9 PMID: 24072823
  5. Pyrrolysine encoded by UAG in Archaea: charging of a UAG-decoding specialized tRNA.
    Science. 2002 May 24;296(5572):1459-62 PMID: 12029131
  6. Identification of nucleotides and amino acids that mediate the interaction between ribosomal protein L30 and the SECIS element.
    BMC Mol Biol. 2013;14:12 PMID: 23777426
  7. In vivo role of three fused corrinoid/methyl transfer proteins in Methanosarcina acetivorans.
    Mol Microbiol. 2009 Jun;72(5):1260-72 PMID: 19432805
  8. Distinct genetic code expansion strategies for selenocysteine and pyrrolysine are reflected in different aminoacyl-tRNA formation systems.
    FEBS Lett. 2010 Jan 21;584(2):342-9 PMID: 19903474
  9. Programming cells by multiplex genome engineering and accelerated evolution.
    Nature. 2009 Aug 13;460(7257):894-8 PMID: 19633652
  10. Recoded organisms engineered to depend on synthetic amino acids.
    Nature. 2015 Feb 5;518(7537):89-93 PMID: 25607356
  11. Selenocysteine, pyrrolysine, and the unique energy metabolism of methanogenic archaea.
    Archaea. 2010;2010. pii: 453642. doi: 10.1155/2010/453642 PMID: 20847933
  12. The many levels of control on bacterial selenoprotein synthesis.
    Biochim Biophys Acta. 2009 Nov;1790(11):1404-14 PMID: 19328835
  13. Synonymous but not the same: the causes and consequences of codon bias.
    Nat Rev Genet. 2011 Jan;12(1):32-42 PMID: 21102527
  14. Mechanism of oxidant-induced mistranslation by threonyl-tRNA synthetase.
    Nucleic Acids Res. 2014 Jun;42(10):6523-31 PMID: 24744241
  15. Identification and characterization of phosphoseryl-tRNA[Ser]Sec kinase.
    Proc Natl Acad Sci U S A. 2004 Aug 31;101(35):12848-53 PMID: 15317934
  16. The prokaryotic selenoproteome.
    EMBO Rep. 2004 May;5(5):538-43 PMID: 15105824
  17. Protein mistranslation protects bacteria against oxidative stress.
    Nucleic Acids Res. 2015 Feb 18;43(3):1740-8 PMID: 25578967
  18. A nonpyrrolysine member of the widely distributed trimethylamine methyltransferase family is a glycine betaine methyltransferase.
    Proc Natl Acad Sci U S A. 2014 Oct 28;111(43):E4668-76 PMID: 25313086
  19. An unusual tRNAThr derived from tRNAHis reassigns in yeast mitochondria the CUN codons to threonine.
    Nucleic Acids Res. 2011 Jun;39(11):4866-74 PMID: 21321019
  20. Emergence of the universal genetic code imprinted in an RNA record.
    Proc Natl Acad Sci U S A. 2006 Nov 28;103(48):18095-100 PMID: 17110438
  21. Transfer RNA mutation and the malleability of the genetic code.
    J Mol Biol. 1994 Feb 4;235(5):1377-80 PMID: 8107079
  22. Loss of editing activity during the evolution of mitochondrial phenylalanyl-tRNA synthetase.
    J Biol Chem. 2005 Nov 18;280(46):38186-92 PMID: 16162501
  23. In vivo contextual requirements for UAG translation as pyrrolysine.
    Mol Microbiol. 2007 Jan;63(1):229-41 PMID: 17140411
  24. Fidelity at the molecular level: lessons from protein synthesis.
    Cell. 2009 Feb 20;136(4):746-62 PMID: 19239893
  25. A novel RNA binding protein, SBP2, is required for the translation of mammalian selenoprotein mRNAs.
    EMBO J. 2000 Jan 17;19(2):306-14 PMID: 10637234
  26. Selenoproteins in Archaea and Gram-positive bacteria.
    Biochim Biophys Acta. 2009 Nov;1790(11):1520-32 PMID: 19344749
  27. Natural and synthetic selenoproteins.
    Curr Opin Chem Biol. 2014 Oct;22:27-34 PMID: 25261915
  28. A facile system for genetic incorporation of two different noncanonical amino acids into one protein in Escherichia coli.
    Angew Chem Int Ed Engl. 2010 Apr 19;49(18):3211-4 PMID: 20340150
  29. Recoding the genetic code with selenocysteine.
    Angew Chem Int Ed Engl. 2014 Jan 3;53(1):319-23 PMID: 24511637
  30. The selenium to selenoprotein pathway in eukaryotes: more molecular partners than anticipated.
    Biochim Biophys Acta. 2009 Nov;1790(11):1415-23 PMID: 19285539
  31. Genetic analysis of selenocysteine biosynthesis in the archaeon Methanococcus maripaludis.
    Mol Microbiol. 2011 Jul;81(1):249-58 PMID: 21564332
  32. Selection of tRNA charging quality control mechanisms that increase mistranslation of the genetic code.
    Nucleic Acids Res. 2013 Jan;41(2):1104-12 PMID: 23222133
  33. Carbon source-dependent expansion of the genetic code in bacteria.
    Proc Natl Acad Sci U S A. 2012 Dec 18;109(51):21070-5 PMID: 23185002
  34. Crystal structure of methylornithine synthase (PylB): insights into the pyrrolysine biosynthesis.
    Angew Chem Int Ed Engl. 2012 Feb 6;51(6):1339-42 PMID: 22095926
  35. The selection-mutation-drift theory of synonymous codon usage.
    Genetics. 1991 Nov;129(3):897-907 PMID: 1752426
  36. Direct charging of tRNA(CUA) with pyrrolysine in vitro and in vivo.
    Nature. 2004 Sep 16;431(7006):333-5 PMID: 15329732
  37. The thioredoxin antioxidant system.
    Free Radic Biol Med. 2014 Jan;66:75-87 PMID: 23899494
  38. Adding new chemistries to the genetic code.
    Annu Rev Biochem. 2010;79:413-44 PMID: 20307192
  39. Precise manipulation of chromosomes in vivo enables genome-wide codon replacement.
    Science. 2011 Jul 15;333(6040):348-53 PMID: 21764749
  40. The human SepSecS-tRNASec complex reveals the mechanism of selenocysteine formation.
    Science. 2009 Jul 17;325(5938):321-5 PMID: 19608919
  41. Codon catalog usage and the genome hypothesis.
    Nucleic Acids Res. 1980 Jan 11;8(1):r49-r62 PMID: 6986610
  42. Upgrading protein synthesis for synthetic biology.
    Nat Chem Biol. 2013 Oct;9(10):594-8 PMID: 24045798
  43. Adaptive translation as a mechanism of stress response and adaptation.
    Annu Rev Genet. 2013;47:121-37 PMID: 23988117
  44. Selenocysteine confers resistance to inactivation by oxidation in thioredoxin reductase: comparison of selenium and sulfur enzymes.
    Biochemistry. 2013 Aug 13;52(32):5472-81 PMID: 23865454
  45. Environmental perturbations lift the degeneracy of the genetic code to regulate protein levels in bacteria.
    Proc Natl Acad Sci U S A. 2013 Feb 5;110(6):2419-24 PMID: 23277573
  46. Codon Bias as a Means to Fine-Tune Gene Expression.
    Mol Cell. 2015 Jul 16;59(2):149-61 PMID: 26186290
  47. Residue-specific incorporation of non-canonical amino acids into proteins: recent developments and applications.
    Curr Opin Chem Biol. 2010 Dec;14(6):774-80 PMID: 21071259
  48. Pyrrolysine analogs as substrates for bacterial pyrrolysyl-tRNA synthetase in vitro and in vivo.
    Biosci Biotechnol Biochem. 2012;76(1):205-8 PMID: 22232266
  49. Reversion of a fungal genetic code alteration links proteome instability with genomic and phenotypic diversification.
    Proc Natl Acad Sci U S A. 2013 Jul 2;110(27):11079-84 PMID: 23776239
  50. Evolving tRNA(Sec) for efficient canonical incorporation of selenocysteine.
    J Am Chem Soc. 2015 Jan 14;137(1):46-9 PMID: 25521771
  51. Highly reproductive Escherichia coli cells with no specific assignment to the UAG codon.
    Sci Rep. 2015;5:9699 PMID: 25982672
  52. Bacteriophages use an expanded genetic code on evolutionary paths to higher fitness.
    Nat Chem Biol. 2014 Mar;10(3):178-80 PMID: 24487692
  53. Revealing the amino acid composition of proteins within an expanded genetic code.
    Nucleic Acids Res. 2015 Jan;43(2):e8 PMID: 25378305
  54. Catalytic properties of an Escherichia coli formate dehydrogenase mutant in which sulfur replaces selenium.
    Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8450-4 PMID: 1924303
  55. N-acetyl lysyl-tRNA synthetases evolved by a CcdB-based selection possess N-acetyl lysine specificity in vitro and in vivo.
    FEBS Lett. 2012 Mar 23;586(6):729-33 PMID: 22289181
  56. Use of the UGA terminator as a tryptophan codon in yeast mitochondria.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3784-5 PMID: 226981
  57. Natural expansion of the genetic code.
    Nat Chem Biol. 2007 Jan;3(1):29-35 PMID: 17173027
  58. Assembly of the mitochondrial membrane system: sequences of yeast mitochondrial valine and an unusual threonine tRNA gene.
    Cell. 1979 Sep;18(1):47-53 PMID: 389433
  59. Agmatine-conjugated cytidine in a tRNA anticodon is essential for AUA decoding in archaea.
    Nat Chem Biol. 2010 Apr;6(4):277-82 PMID: 20139989
  60. Mycobacterial mistranslation is necessary and sufficient for rifampicin phenotypic resistance.
    Proc Natl Acad Sci U S A. 2014 Jan 21;111(3):1132-7 PMID: 24395793
  61. A novel lysine-substituted nucleoside in the first position of the anticodon of minor isoleucine tRNA from Escherichia coli.
    J Biol Chem. 1988 Jul 5;263(19):9261-7 PMID: 3132458
  62. A dynamic competition between release factor 2 and the tRNA(Sec) decoding UGA at the recoding site of Escherichia coli formate dehydrogenase H.
    EMBO J. 2001 Dec 17;20(24):7284-93 PMID: 11743004
  63. Comparative genomics highlights the unique biology of Methanomassiliicoccales, a Thermoplasmatales-related seventh order of methanogenic archaea that encodes pyrrolysine.
    BMC Genomics. 2014;15:679 PMID: 25124552
  64. Aminoacyl-tRNA synthesis and translational quality control.
    Annu Rev Microbiol. 2009;63:61-78 PMID: 19379069
  65. Pyrrolysine is not hardwired for cotranslational insertion at UAG codons.
    Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3141-6 PMID: 17360621
  66. Total synthesis of a functional designer eukaryotic chromosome.
    Science. 2014 Apr 4;344(6179):55-8 PMID: 24674868
  67. Studies on polynucleotides, XLIX. Stimulation of the binding of aminoacyl-sRNA's to ribosomes by ribotrinucleotides and a survey of codon assignments for 20 amino acids.
    Proc Natl Acad Sci U S A. 1965 Nov;54(5):1378-85 PMID: 5325653
  68. Normal tRNAs promote ribosomal frameshifting.
    Cell. 1979 Dec;18(4):1119-31 PMID: 391405
  69. Polyspecific pyrrolysyl-tRNA synthetases from directed evolution.
    Proc Natl Acad Sci U S A. 2014 Nov 25;111(47):16724-9 PMID: 25385624
  70. The path of lysine to pyrrolysine.
    Curr Opin Chem Biol. 2013 Aug;17(4):619-25 PMID: 23856058
  71. Expanding and reprogramming the genetic code of cells and animals.
    Annu Rev Biochem. 2014;83:379-408 PMID: 24555827
  72. Ribosome fidelity: tRNA discrimination, proofreading and induced fit.
    Trends Biochem Sci. 2001 Feb;26(2):124-30 PMID: 11166571
  73. Transcriptional Response of Selenopolypeptide Genes and Selenocysteine Biosynthesis Machinery Genes in Escherichia coli during Selenite Reduction.
    Int J Microbiol. 2014;2014:394835 PMID: 24839442
  74. The appearance of pyrrolysine in tRNAHis guanylyltransferase by neutral evolution.
    Proc Natl Acad Sci U S A. 2009 Dec 15;106(50):21103-8 PMID: 19965368
  75. Reprogramming the genetic code.
    EMBO J. 2011 Jun 15;30(12):2312-24 PMID: 21602790
  76. Structural basis for mRNA recognition by elongation factor SelB.
    Nat Struct Mol Biol. 2005 Feb;12(2):198-203 PMID: 15665870
  77. The distinction between recoding and codon reassignment.
    Genetics. 2010 Aug;185(4):1535-6 PMID: 20713743
  78. Engineering the elongation factor Tu for efficient selenoprotein synthesis.
    Nucleic Acids Res. 2014 Sep;42(15):9976-83 PMID: 25064855
  79. Yeast mitochondrial threonyl-tRNA synthetase recognizes tRNA isoacceptors by distinct mechanisms and promotes CUN codon reassignment.
    Proc Natl Acad Sci U S A. 2012 Feb 28;109(9):3281-6 PMID: 22343532
  80. Genetic code supports targeted insertion of two amino acids by one codon.
    Science. 2009 Jan 9;323(5911):259-61 PMID: 19131629
  81. A synthetic tRNA for EF-Tu mediated selenocysteine incorporation in vivo and in vitro.
    FEBS Lett. 2015 Aug 4;589(17):2194-9 PMID: 26160755
  82. C to U editing of the anticodon of imported mitochondrial tRNA(Trp) allows decoding of the UGA stop codon in Leishmania tarentolae.
    EMBO J. 1999 Dec 15;18(24):7056-62 PMID: 10601027
  83. Characterization and evolutionary history of an archaeal kinase involved in selenocysteinyl-tRNA formation.
    Nucleic Acids Res. 2008 Mar;36(4):1247-59 PMID: 18174226
  84. Biochemical and genetic analysis of Salmonella typhimurium and Escherichia coli mutants defective in specific incorporation of selenium into formate dehydrogenase and tRNAs.
    Biofactors. 1989 Mar;2(1):35-44 PMID: 2679652
  85. The mechanisms of codon reassignments in mitochondrial genetic codes.
    J Mol Evol. 2007 Jun;64(6):662-88 PMID: 17541678
  86. Codon reading patterns in Drosophila melanogaster mitochondria based on their tRNA sequences: a unique wobble rule in animal mitochondria.
    Nucleic Acids Res. 1999 Nov 1;27(21):4291-7 PMID: 10518623
  87. Release factor one is nonessential in Escherichia coli.
    ACS Chem Biol. 2012 Aug 17;7(8):1337-44 PMID: 22662873
  88. Stop codon reassignments in the wild.
    Science. 2014 May 23;344(6186):909-13 PMID: 24855270
  89. Selenocysteine.
    Annu Rev Biochem. 1996;65:83-100 PMID: 8811175
  90. Biocontainment of genetically modified organisms by synthetic protein design.
    Nature. 2015 Feb 5;518(7537):55-60 PMID: 25607366
  91. Characterization of a Methanosarcina acetivorans mutant unable to translate UAG as pyrrolysine.
    Mol Microbiol. 2006 Jan;59(1):56-66 PMID: 16359318
  92. Inactivation of the selB gene in Methanococcus maripaludis: effect on synthesis of selenoproteins and their sulfur-containing homologs.
    J Bacteriol. 2003 Jan;185(1):107-14 PMID: 12486046
  93. Peroxidase activity of selenoprotein GrdB of glycine reductase and stabilisation of its integrity by components of proprotein GrdE from Eubacterium acidaminophilum.
    Arch Microbiol. 2007 Jan;187(1):29-43 PMID: 17009022
  94. Expanded use of sense codons is regulated by modified cytidines in tRNA.
    Proc Natl Acad Sci U S A. 2013 Jul 2;110(27):10964-9 PMID: 23781103
  95. Eukaryotic initiation factor 4a3 is a selenium-regulated RNA-binding protein that selectively inhibits selenocysteine incorporation.
    Mol Cell. 2009 Aug 28;35(4):479-89 PMID: 19716792
  96. Reducing the genetic code induces massive rearrangement of the proteome.
    Proc Natl Acad Sci U S A. 2014 Dec 2;111(48):17206-11 PMID: 25404328
  97. Pyrrolysyl-tRNA synthetase-tRNA(Pyl) structure reveals the molecular basis of orthogonality.
    Nature. 2009 Feb 26;457(7233):1163-7 PMID: 19118381
  98. Agmatidine, a modified cytidine in the anticodon of archaeal tRNA(Ile), base pairs with adenosine but not with guanosine.
    Proc Natl Acad Sci U S A. 2010 Feb 16;107(7):2872-7 PMID: 20133752
  99. Cell biology: A fable of too much too fast.
    Nature. 2013 Mar 7;495(7439):57-8 PMID: 23417066
  100. Different catalytic mechanisms in mammalian selenocysteine- and cysteine-containing methionine-R-sulfoxide reductases.
    PLoS Biol. 2005 Dec;3(12):e375 PMID: 16262444
  101. Formate dehydrogenase--a versatile enzyme in changing environments.
    Curr Opin Struct Biol. 2003 Aug;13(4):418-23 PMID: 12948771
  102. Genomically recoded organisms expand biological functions.
    Science. 2013 Oct 18;342(6156):357-60 PMID: 24136966
  103. Genetically encoding N(epsilon)-acetyllysine in recombinant proteins.
    Nat Chem Biol. 2008 Apr;4(4):232-4 PMID: 18278036
  104. Structure of the unusual seryl-tRNA synthetase reveals a distinct zinc-dependent mode of substrate recognition.
    EMBO J. 2006 Jun 7;25(11):2498-509 PMID: 16675947
  105. RNA codewords and protein synthesis, VII. On the general nature of the RNA code.
    Proc Natl Acad Sci U S A. 1965 May;53(5):1161-8 PMID: 5330357
  106. Decameric SelA•tRNA(Sec) ring structure reveals mechanism of bacterial selenocysteine formation.
    Science. 2013 Apr 5;340(6128):75-8 PMID: 23559248
  107. Sounds of silence: synonymous nucleotides as a key to biological regulation and complexity.
    Nucleic Acids Res. 2013 Feb 1;41(4):2073-94 PMID: 23293005
  108. Radiochemical assay of glutathione S-epoxide transferase and its enhancement by phenobarbital in rat liver in vivo.
    Biochem Pharmacol. 1975 Sep 1;24(17):1569-72 PMID: 9
  109. A serine sensor for multicellularity in a bacterium.
    Elife. 2013;2:e01501 PMID: 24347549
  110. Creation of a bacterial cell controlled by a chemically synthesized genome.
    Science. 2010 Jul 2;329(5987):52-6 PMID: 20488990
  111. UGA is an additional glycine codon in uncultured SR1 bacteria from the human microbiota.
    Proc Natl Acad Sci U S A. 2013 Apr 2;110(14):5540-5 PMID: 23509275
  112. How an obscure archaeal gene inspired the discovery of selenocysteine biosynthesis in humans.
    IUBMB Life. 2009 Jan;61(1):35-9 PMID: 18798524
  113. An aminoacyl-tRNA synthetase that specifically activates pyrrolysine.
    Proc Natl Acad Sci U S A. 2004 Aug 24;101(34):12450-4 PMID: 15314242
  114. Codon reassignment in the Escherichia coli genetic code.
    Nucleic Acids Res. 2010 Dec;38(22):8188-95 PMID: 20702426
  115. Candida albicans CUG mistranslation is a mechanism to create cell surface variation.
    MBio. 2013;4(4). pii: e00285-13. doi: 10.1128/mBio.00285-13 PMID: 23800396
  116. Structure of pyrrolysyl-tRNA synthetase, an archaeal enzyme for genetic code innovation.
    Proc Natl Acad Sci U S A. 2007 Jul 3;104(27):11268-73 PMID: 17592110
  117. Non-optimal codon usage is a mechanism to achieve circadian clock conditionality.
    Nature. 2013 Mar 7;495(7439):116-20 PMID: 23417065
  118. Multiplex genome engineering using CRISPR/Cas systems.
    Science. 2013 Feb 15;339(6121):819-23 PMID: 23287718
  119. Wobble decoding by the Escherichia coli selenocysteine insertion machinery.
    Nucleic Acids Res. 2013 Nov;41(21):9800-11 PMID: 23982514
  120. Enhanced phosphoserine insertion during Escherichia coli protein synthesis via partial UAG codon reassignment and release factor 1 deletion.
    FEBS Lett. 2012 Oct 19;586(20):3716-22 PMID: 22982858
  121. PylSn and the homologous N-terminal domain of pyrrolysyl-tRNA synthetase bind the tRNA that is essential for the genetic encoding of pyrrolysine.
    J Biol Chem. 2012 Sep 21;287(39):32738-46 PMID: 22851181
  122. Innate immune and chemically triggered oxidative stress modifies translational fidelity.
    Nature. 2009 Nov 26;462(7272):522-6 PMID: 19940929
  123. Translation termination in pyrrolysine-utilizing archaea.
    FEBS Lett. 2009 Nov 3;583(21):3455-60 PMID: 19796638
  124. The anti-Shine-Dalgarno sequence drives translational pausing and codon choice in bacteria.
    Nature. 2012 Apr 26;484(7395):538-41 PMID: 22456704
  125. Dynamic evolution of selenocysteine utilization in bacteria: a balance between selenoprotein loss and evolution of selenocysteine from redox active cysteine residues.
    Genome Biol. 2006;7(10):R94 PMID: 17054778
  126. Natural reassignment of CUU and CUA sense codons to alanine in Ashbya mitochondria.
    Nucleic Acids Res. 2014 Jan;42(1):499-508 PMID: 24049072
  127. RNA-guided genetic silencing systems in bacteria and archaea.
    Nature. 2012 Feb 16;482(7385):331-8 PMID: 22337052
  128. A new UAG-encoded residue in the structure of a methanogen methyltransferase.
    Science. 2002 May 24;296(5572):1462-6 PMID: 12029132
  129. The complete biosynthesis of the genetically encoded amino acid pyrrolysine from lysine.
    Nature. 2011 Mar 31;471(7340):647-50 PMID: 21455182
  130. Evolutionary dynamics of eukaryotic selenoproteomes: large selenoproteomes may associate with aquatic life and small with terrestrial life.
    Genome Biol. 2007;8(9):R198 PMID: 17880704
  131. The Croonian lecture, 1966. The genetic code.
    Proc R Soc Lond B Biol Sci. 1967 Apr 18;167(1009):331-47 PMID: 4382798
  132. Genome sequence of "Candidatus Methanomethylophilus alvus" Mx1201, a methanogenic archaeon from the human gut belonging to a seventh order of methanogens.
    J Bacteriol. 2012 Dec;194(24):6944-5 PMID: 23209209
  133. Trends in selenium utilization in marine microbial world revealed through the analysis of the global ocean sampling (GOS) project.
    PLoS Genet. 2008 Jun;4(6):e1000095 PMID: 18551170
  134. Non-optimal codon usage affects expression, structure and function of clock protein FRQ.
    Nature. 2013 Mar 7;495(7439):111-5 PMID: 23417067
  135. Selenoproteins-What unique properties can arise with selenocysteine in place of cysteine?
    Exp Cell Res. 2010 May 1;316(8):1296-303 PMID: 20206159
  136. The accuracy of codon recognition by polypeptide release factors.
    Proc Natl Acad Sci U S A. 2000 Feb 29;97(5):2046-51 PMID: 10681447
  137. Reassignment of a rare sense codon to a non-canonical amino acid in Escherichia coli.
    Nucleic Acids Res. 2015 Sep 18;43(16):8111-22 PMID: 26240376
  138. Pyrrolysine analogues as substrates for pyrrolysyl-tRNA synthetase.
    FEBS Lett. 2006 Dec 11;580(28-29):6695-700 PMID: 17126325
  139. Function of genetically encoded pyrrolysine in corrinoid-dependent methylamine methyltransferases.
    Curr Opin Chem Biol. 2004 Oct;8(5):484-91 PMID: 15450490
  140. Rewiring translation for elongation factor Tu-dependent selenocysteine incorporation.
    Angew Chem Int Ed Engl. 2013 Jan 28;52(5):1441-5 PMID: 23193031
  141. Expanded genetic code technologies for incorporating modified lysine at multiple sites.
    Chembiochem. 2014 Oct 13;15(15):2181-7 PMID: 25179816
  142. Naturally occurring aminoacyl-tRNA synthetases editing-domain mutations that cause mistranslation in Mycoplasma parasites.
    Proc Natl Acad Sci U S A. 2011 Jun 7;108(23):9378-83 PMID: 21606343
Article Info
Journal
Nature reviews. Microbiology
Abbr.
Nat Rev Microbiol
ISSN
1740-1534
DOI
10.1038/nrmicro3568
Published
2015-11-00
Epub
2015-00-22
Pages
707-721
Language
English
Region
England
NLM ID
101190261
PMCID
PMC4712924
Subset
IM
Grants
Canadian Institutes of Health Research · 950‑229917 · Canada
NIGMS NIH HHS · R37 GM022854 · United States
NIGMS NIH HHS · R01 GM022854 · United States
NIGMS NIH HHS · R01 GM115431 · United States
NIGMS NIH HHS · GM022854 · United States
NIGMS NIH HHS · GM115431 · United States
External Links
PubMed source DOI: 10.1038/nrmicro3568 PMC Full Text View journal details
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