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PMID: 2659530 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

T-cell recognition of the 18-kilodalton antigen of Mycobacterium leprae.

Infection and immunity ·Vol. 57 ·No. 7 ·1989-07-00 ·Pages 1979-83

Dockrell HM, Stoker NG, Lee SP, Jackson M, Grant KA, Jouy NF, Lucas SB, Hasan R, Hussain R, McAdam KP

Abstract

The 18-kilodalton (kDa) antigen of Mycobacterium leprae was expressed as a fusion protein with a 2-kDa leader peptide and used in proliferation assays with peripheral blood cells. Fifty percent of untreated tuberculoid leprosy patients and 93% of long-term leprosy contacts responded to the recombinant protein in lymphocyte transformation tests. Comparison of the stimulation indices in the two groups showed that the contacts responded more strongly than the tuberculoid leprosy patients. Seventy percent of Mycobacterium bovis BCG-vaccinated European donors responded, although with low stimulation indices. The isolation of 18-kDa antigen-responsive T-cell lines from a BCG-vaccinated British donor confirmed that the 18-kDa antigen contains at least one cross-reactive epitope. These results indicate that the 18-kDa protein is an important antigen in the immune response to leprosy.

MeSH Terms
Amino Acid Sequence Antigens, Bacterial/analysis BCG Vaccine/immunology Carrier State/immunology Cell Line Cell Separation Female Humans Leprosy/immunology Male Molecular Sequence Data Molecular Weight Mycobacterium leprae/immunology Protein Sorting Signals/immunology Recombinant Fusion Proteins/immunology Sonication T-Lymphocytes/immunology
Chemicals
Antigens, Bacterial BCG Vaccine Protein Sorting Signals Recombinant Fusion Proteins
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Dockrell H M
Department of Clinical Sciences, London School of Hygiene and Tropical Medicine, United Kingdom.
Stoker N G
Lee S P
Jackson M
Grant K A
Jouy N F
Lucas S B
Hasan R
Hussain R
McAdam K P
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.57.7.1979-1983.1989
Published
1989-07-00
Pages
1979-83
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC313830
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1128/iai.57.7.1979-1983.1989 PMC Full Text View journal details
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