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PMID: 2721497 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Interaction of nuclear factors with the upstream region of the alpha-subunit gene of chicken muscle acetylcholine receptor: variations with muscle differentiation and denervation.

The EMBO journal ·Vol. 8 ·No. 3 ·1989-03-00 ·Pages 687-94

Piette J, Klarsfeld A, Changeux JP

Abstract

The region lying between nucleotides (nt) -110 and -45 of chicken acetylcholine receptor alpha-subunit gene 5' upstream sequence confers developmental control of expression in primary cultures of chicken myotubes. This region interacts with several nuclear factors present in muscle cells as shown by DNase I footprinting and gel-retardation experiments. An Sp1-like factor and a guanine stretch-binding protein were found to bind to overlapping sites immediately upstream of the TATA box. Several factors interacting in the same region with a domain similar to the SV40 enhancer core appeared during in vitro differentiation of myoblasts into myotubes. The concentration of some of these factors increased also after denervation of leg muscle in newborn chickens. The specific interaction of nuclear factors with this domain may thus play a critical role in the regulation of alpha-subunit gene expression by muscle differentiation and electrical activity.

MeSH Terms
Animals Base Sequence Binding Sites Chickens Culture Techniques DNA/genetics,metabolism DNA-Binding Proteins/metabolism Gene Expression Regulation Molecular Sequence Data Muscle Denervation Muscle Development Muscles/innervation,metabolism Nuclear Proteins/metabolism Protein Conformation Receptors, Cholinergic/genetics
Chemicals
DNA-Binding Proteins Nuclear Proteins Receptors, Cholinergic DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Piette J
URA CNRS 0210, Département des Biotechnologies, Institut Pasteur, Paris, France.
Klarsfeld A
Changeux J P
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-03-00
Pages
687-94
Language
English
Region
England
NLM ID
8208664
PMCID
PMC400862
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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