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PMID: 2721504 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The integrated conjugative plasmid pSAM2 of Streptomyces ambofaciens is related to temperate bacteriophages.

The EMBO journal ·Vol. 8 ·No. 3 ·1989-03-00 ·Pages 973-80

Boccard F, Smokvina T, Pernodet JL, Friedmann A, Guérineau M

Abstract

Streptomyces ambofaciens ATCC23877 and derivatives contain the 11-kb element pSAM2 present in an integrated state or as a free and integrated plasmid. This element, able to integrate site-specifically in the genome of different Streptomyces species, is conjugative and mobilizes chromosomal markers. Besides these plasmid functions, we have shown that the site-specific recombination system of pSAM2 presents strong similarities with that of several temperate phages. The integration event is promoted by a site-specific recombinase of the integrase family. The int gene encoding this integrase is closely linked to the plasmid attachment site (attP). A small open reading frame (ORF) overlaps the int gene and the predicted protein exhibits similarities with Xis proteins involved in phages excision. The integrated copy of pSAM2 in strain ATCC23877 is flanked by att sequences (attL and attR). Another att sequence (attX) is present in this strain and attX and attL are the boundaries of a 42-kb fragment (xSAM1) absent, as well as pSAM2, from S.ambofaciens DSM40697. Sequences partially similar to pSAM2 int gene are found near the chromosomal integration zone in both S.ambofaciens strains. The possible origin of pSAM2, an element carrying plasmid as well as phage features, is discussed.

MeSH Terms
Amino Acid Sequence Attachment Sites, Microbiological Bacteriophages/genetics Base Sequence Conjugation, Genetic DNA Nucleotidyltransferases/genetics DNA, Bacterial/genetics Genes, Bacterial Integrases Molecular Sequence Data Plasmids Recombination, Genetic Restriction Mapping Streptomyces/genetics
Chemicals
DNA, Bacterial DNA Nucleotidyltransferases Integrases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Boccard F
Laboratoire de Biologie et Génétique Moléculaire, URA CNRS D0086, Université Paris-Sud, Orsay, France.
Smokvina T
Pernodet J L
Friedmann A
Guérineau M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-03-00
Pages
973-80
Language
English
Region
England
NLM ID
8208664
PMCID
PMC400899
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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