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PMID: 2842151 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Structural and functional analysis of Tn4430: identification of an integrase-like protein involved in the co-integrate-resolution process.

The EMBO journal ·Vol. 7 ·No. 5 ·1988-05-00 ·Pages 1515-26

Mahillon J, Lereclus D

Abstract

The 4149-bp transposon Tn4430 from Bacillus thuringiensis is delineated by 38-bp inverted repeats and codes for a 113-kd protein that shares homology with the transposases (TnpA) of Tn3, Tn21 and Tn501. Through transpositional recombination, this protein generates the formation of co-integrates between both donor and target replicons, with duplication of Tn4430 molecules. These features are characteristic of transposons of the Tn3 family (class II elements). The second step of the transposition process, the co-integrate resolution, is mediated by a 32-kd protein. This protein (TnpI) displays regional similarities with site-specific recombinases of the integrase family, such as Int of bacteriophage lambda, Cre of bacteriophage P1 or TnpA and TnpB of the Tn554 transposon. Moreover, the 250-bp sequence upstream to the tnpI gene contains several structural features that are reminiscent of the attP attachment site of phage lambda. This unique association between the integrase-like TnpI recombinase and the TnpA transposase qualifies Tn4430 as a member of a new group within the class II mobile genetic elements.

MeSH Terms
Amino Acid Sequence Bacillus thuringiensis/enzymology,genetics Base Sequence DNA Nucleotidyltransferases/genetics,metabolism DNA Transposable Elements DNA, Bacterial/genetics Escherichia coli/enzymology,genetics Integrases Molecular Sequence Data Nucleotidyltransferases/genetics,metabolism Recombination, Genetic Transposases
Chemicals
DNA Transposable Elements DNA, Bacterial DNA Nucleotidyltransferases Integrases Nucleotidyltransferases Transposases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mahillon J
Plant Genetic Systems, Gent, Belgium.
Lereclus D
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49 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-05-00
Pages
1515-26
Language
English
Region
England
NLM ID
8208664
PMCID
PMC458404
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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