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PMID: 2734288 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Conserved terminal hairpin sequences of histone mRNA precursors are not involved in duplex formation with the U7 RNA but act as a target site for a distinct processing factor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 12 ·1989-06-00 ·Pages 4345-9

Vasserot AP, Schaufele FJ, Birnstiel ML

Abstract

The hairpin loop structure and the downstream spacer element of histone mRNA precursors are both needed for efficient 3' end formation in vivo and in vitro. Though generally considered as a single processing signal, these two motifs are involved in different types of interaction with the processing machinery. Whereas RNA duplex formation between the downstream spacer element and the U7 small nuclear RNA is essential for processing, we show here that base pairing between the histone stem-loop structure and the U7 RNA is not relevant. Our experiments demonstrate that a processing factor other than the U7 RNA makes contact with the highly conserved hairpin structure of the histone precursor. The recognition of the target site by the processing factor is structure and sequence specific. Prevention of this interaction results in an 80% decrease of 3' cleavage efficiency in vitro. The hairpin binding factor is Sm-precipitable and can be partially separated from the U7 small nuclear ribonucleoprotein particle on a Mono Q column.

MeSH Terms
Animals Base Sequence Embryo, Nonmammalian Genes Histones/genetics Molecular Sequence Data Mutation Nucleic Acid Conformation RNA Precursors/genetics,ultrastructure RNA, Small Nuclear/genetics Sea Urchins Transcription, Genetic
Chemicals
Histones RNA Precursors RNA, Small Nuclear
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Vasserot A P
Research Institute of Molecular Pathology, Vienna, Austria.
Schaufele F J
Birnstiel M L
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.12.4345
Published
1989-06-00
Pages
4345-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC287265
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.86.12.4345 PMC Full Text View journal details
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