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PMID: 3380087 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Structural and functional characterization of mouse U7 small nuclear RNA active in 3' processing of histone pre-mRNA.

Molecular and cellular biology ·Vol. 8 ·No. 4 ·1988-04-00 ·Pages 1518-24

Soldati D, Schümperli D

Abstract

Oligonucleotides derived from the spacer element of the histone RNA 3' processing signal were used to characterize mouse U7 small nuclear RNA (snRNA), i.e., the snRNA component active in 3' processing of histone pre-mRNA. Under RNase H conditions, such oligonucleotides inhibited the processing reaction, indicating the formation of a DNA-RNA hybrid with a functional ribonucleoprotein component. Moreover, these oligonucleotides hybridized to a single nuclear RNA species of approximately 65 nucleotides. The sequence of this RNA was determined by primer extension experiments and was found to bear several structural similarities with sea urchin U7 snRNA. The comparison of mouse and sea urchin U7 snRNA structures yields some further insight into the mechanism of histone RNA 3' processing.

MeSH Terms
Animals Base Sequence Cell Nucleus/metabolism DNA, Ribosomal/genetics Histones/genetics Mice Molecular Sequence Data Nucleic Acid Conformation Nucleic Acid Hybridization RNA Precursors/genetics RNA Processing, Post-Transcriptional RNA, Small Nuclear/genetics Sea Urchins Sequence Homology, Nucleic Acid
Chemicals
DNA, Ribosomal Histones RNA Precursors RNA, Small Nuclear
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Soldati D
Institut für Molekularbiologie II, Universität Zürich, Switzerland.
Schümperli D
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.4.1518-1524.1988
Published
1988-04-00
Pages
1518-24
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363311
Subset
IM
Databases
GENBANK
M18341, M18675
External Links
PubMed source DOI: 10.1128/mcb.8.4.1518-1524.1988 PMC Full Text View journal details
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