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PMID: 2771954 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Principal neutralizing domain of the human immunodeficiency virus type 1 envelope protein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 17 ·1989-09-00 ·Pages 6768-72

Javaherian K, Langlois AJ, McDanal C, Ross KL, Eckler LI, Jellis CL, Profy AT, Rusche JR, Bolognesi DP, Putney SD

Abstract

The principal neutralizing determinant of human immunodeficiency virus type 1 (HIV-1) is located in the external envelope protein, gp120, and has previously been mapped to a 24-amino acid-long sequence (denoted RP135). We show here that deletion of this sequence renders the envelope unable to elicit neutralizing antibodies. In addition, using synthetic peptide fragments of RP135, we have mapped the neutralizing determinant to 8 amino acids and found that a peptide of this size elicits neutralizing antibodies. This sequence contains a central Gly-Pro-Gly that is generally conserved between different HIV-1 isolates and is flanked by amino acids that differ from isolate to isolate. Antibodies elicited by peptides from one isolate do not neutralize two different isolates, and a hybrid peptide, consisting of amino acid sequences from two isolates, elicits neutralizing antibodies to both isolates. By using a mixture of peptides of this domain or a mixture of such hybrid peptides the type-specificity of the neutralizing antibody response to this determinant can perhaps be overcome.

MeSH Terms
Amino Acid Sequence Disulfides Genes Genes, Viral HIV-1/genetics,immunology Molecular Sequence Data Neutralization Tests Recombinant Proteins/immunology,isolation & purification Restriction Mapping Viral Envelope Proteins/genetics,immunology,isolation & purification
Chemicals
Disulfides Recombinant Proteins Viral Envelope Proteins
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Javaherian K
Repligen Corporation, Cambridge, MA 02139.
Langlois A J
McDanal C
Ross K L
Eckler L I
Jellis C L
Profy A T
Rusche J R
Bolognesi D P
Putney S D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.17.6768
Published
1989-09-00
Pages
6768-72
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC297927
Subset
IM
Grants
NCI NIH HHS · 5PO1-CA43447-04 · United States
NIH HHS · FOD-0758 · United States
External Links
PubMed source DOI: 10.1073/pnas.86.17.6768 PMC Full Text View journal details
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