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PMID: 2780558 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Different 3'-end processing produces two independently regulated mRNAs from a single H1 histone gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 18 ·1989-09-00 ·Pages 7002-6

Cheng GH, Nandi A, Clerk S, Skoultchi AI

Abstract

We describe the isolation of a mouse H1 histone gene that encodes two mRNA transcripts. One mRNA ends just beyond the coding region, near a highly conserved palindrome sequence typical of cell cycle-regulated histone genes. The level of this transcript is coupled to DNA replication. The second mRNA ends nearly 1 kilobase downstream near a polyadenylation signal. This mRNA is polyadenylylated, and its accumulation is not coupled to DNA replication. The two mRNAs are regulated independently and in some circumstances in opposite directions under several physiological conditions. The production of a polyadenylylated mRNA from an otherwise cell cycle-regulated histone gene may allow for continued synthesis of the histone protein when DNA synthesis ceases in nondividing cells.

MeSH Terms
Acetamides/pharmacology Amino Acid Sequence Animals Base Sequence Cell Cycle Cell Line DNA, Neoplasm/genetics,isolation & purification Gene Expression Regulation Genes/drug effects Genetic Variation Histones/genetics Hydroxyurea/pharmacology Leukemia, Erythroblastic, Acute Mice Molecular Sequence Data RNA, Messenger/genetics Transcription, Genetic/drug effects
Chemicals
Acetamides DNA, Neoplasm Histones RNA, Messenger hexamethylene bisacetamide Hydroxyurea
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cheng G H
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461.
Nandi A
Clerk S
Skoultchi A I
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.18.7002
Published
1989-09-00
Pages
7002-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC297980
Subset
IM
Grants
NCI NIH HHS · CA13330 · United States
NCI NIH HHS · CA16368 · United States
Databases
GENBANK
M25365
External Links
PubMed source DOI: 10.1073/pnas.86.18.7002 PMC Full Text View journal details
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