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PMID: 2832736 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Expression of a Drosophila melanogaster acetylcholine receptor-related gene in the central nervous system.

Molecular and cellular biology ·Vol. 8 ·No. 2 ·1988-02-00 ·Pages 778-85

Wadsworth SC, Rosenthal LS, Kammermeyer KL, Potter MB, Nelson DJ

Abstract

We isolated Drosophila melanogaster genomic sequences with nucleotide and amino acid sequence homology to subunits of vertebrate acetylcholine receptor by hybridization with a Torpedo acetylcholine receptor subunit cDNA probe. Five introns are present in the portion of the Drosophila gene encoding the unprocessed protein and are positionally conserved relative to the human acetylcholine receptor alpha-subunit gene. The Drosophila genomic clone hybridized to salivary gland polytene chromosome 3L within region 64B and was termed AChR64B. A 3-kilobase poly(A)-containing transcript complementary to the AChR64B clone was readily detectable by RNA blot hybridizations during midembryogenesis, during metamorphosis, and in newly enclosed adults. AChR64B transcripts were localized to the cellular regions of the central nervous system during embryonic, larval, pupal, and adult stages of development. During metamorphosis, a temporal relationship between the morphogenesis of the optic lobe and expression of AChR64B transcripts was observed.

MeSH Terms
Amino Acid Sequence Animals Base Sequence DNA Restriction Enzymes Drosophila melanogaster/embryology,genetics,growth & development Embryo, Nonmammalian/metabolism Genes Humans Larva Molecular Sequence Data Nervous System/growth & development Pupa Receptors, Cholinergic/genetics Sequence Homology, Nucleic Acid Transcription, Genetic
Chemicals
Receptors, Cholinergic DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Wadsworth S C
Cell Biology Group, Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545.
Rosenthal L S
Kammermeyer K L
Potter M B
Nelson D J
References (29)
29 references, click to expand
  1. Acetylcholine receptors in the central nervous system of Drosophila melanogaster.
    Exp Cell Res. 1978 Jan;111(1):185-90 PMID: 413721
  2. Primary sequence and developmental expression of a novel Drosophila melanogaster src gene.
    Mol Cell Biol. 1987 Jun;7(6):2119-27 PMID: 3110602
  3. Expression of Drosophila epidermal growth factor receptor homologue in mitotic cell populations.
    Development. 1987 Jun;100(2):201-10 PMID: 2443332
  4. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.
    Proc Natl Acad Sci U S A. 1985 Feb;82(4):1074-8 PMID: 3156376
  5. Mapping of brain areas expressing RNA homologous to two different acetylcholine receptor alpha-subunit cDNAs.
    Proc Natl Acad Sci U S A. 1986 Jun;83(11):4076-80 PMID: 3012549
  6. The isolation of structural genes from libraries of eucaryotic DNA.
    Cell. 1978 Oct;15(2):687-701 PMID: 719759
  7. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
    Gene. 1982 Oct;19(3):269-76 PMID: 6295880
  8. Immunological method for mapping genes on Drosophila polytene chromosomes.
    Proc Natl Acad Sci U S A. 1982 Jul;79(14):4381-5 PMID: 6812046
  9. The nicotinic cholinergic receptor: correlation of molecular structure with functional properties.
    Annu Rev Biochem. 1982;51:491-530 PMID: 7051962
  10. Molecular cloning of the acetylcholine receptor.
    Cold Spring Harb Symp Quant Biol. 1983;48 Pt 1:71-8 PMID: 6202451
  11. Acetylcholine receptor structure, function, and evolution.
    Annu Rev Cell Biol. 1985;1:317-51 PMID: 2435309
  12. Patterns of cell division and cell movement in the formation of the imaginal nervous system in Drosophila melanogaster.
    Dev Biol. 1978 Aug;65(2):296-321 PMID: 98369
  13. Maternal inheritance of transcripts from three Drosophila src-related genes.
    Nucleic Acids Res. 1985 Mar 25;13(6):2153-70 PMID: 3923437
  14. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  15. Localization of antigenic determinants in whole Drosophila embryos.
    Dev Biol. 1983 Sep;99(1):261-4 PMID: 6194030
  16. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  17. Characterization of an alpha-bungarotoxin binding component from Drosophila melanogaster.
    J Neurochem. 1977 Dec;29(6):1013-29 PMID: 413880
  18. Transcripts of the six Drosophila actin genes accumulate in a stage- and tissue-specific manner.
    Cell. 1983 May;33(1):115-23 PMID: 6432334
  19. Isolation of a cDNA clone coding for a possible neural nicotinic acetylcholine receptor alpha-subunit.
    Nature. 1986 Jan 30-Feb 5;319(6052):368-74 PMID: 3753746
  20. An improved in situ hybridization method for the detection of cellular RNAs in Drosophila tissue sections and its application for localizing transcripts of the homeotic Antennapedia gene complex.
    EMBO J. 1983;2(4):617-23 PMID: 16453446
  21. Cloning and sequence analysis of calf cDNA and human genomic DNA encoding alpha-subunit precursor of muscle acetylcholine receptor.
    Nature. 1983 Oct 27-Nov 2;305(5937):818-23 PMID: 6688857
  22. Dissociated neurons from normal and mutant Drosophila larval central nervous system in cell culture.
    J Neurosci. 1983 Sep;3(9):1888-99 PMID: 6310066
  23. Quantitation of junctional and extrajunctional acetylcholine receptors by electron microscope autoradiography after 125I-alpha-bungarotoxin binding at mouse neuromuscular junctions.
    J Cell Biol. 1976 Apr;69(1):144-58 PMID: 1254640
  24. Cholinergic neurochemical development of normal and deafferented antennal lobes during metamorphosis of the moth, Manduca sexta.
    Brain Res. 1977 Jan 7;119(2):389-402 PMID: 830392
  25. Primary structure of a developmentally regulated nicotinic acetylcholine receptor protein from Drosophila.
    EMBO J. 1986 Jul;5(7):1503-8 PMID: 16453690
  26. Molecular forms and subunit structure of the acetylcholine receptor in the central nervous system of insects.
    J Neurosci. 1985 Dec;5(12):3386-92 PMID: 4078632
  27. Detection of mrnas in sea urchin embryos by in situ hybridization using asymmetric RNA probes.
    Dev Biol. 1984 Feb;101(2):485-502 PMID: 6692991
  28. Cloning of Drosophila choline acetyltransferase cDNA.
    Proc Natl Acad Sci U S A. 1986 Jun;83(11):4081-5 PMID: 3086876
  29. Nucleic acid hybridization to the DNA of cytological preparations.
    Methods Cell Biol. 1975;10:1-16 PMID: 1102840
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.2.778-785.1988
Published
1988-02-00
Pages
778-85
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363204
Subset
IM
Grants
NCI NIH HHS · CA12708 · United States
NCI NIH HHS · CA35911 · United States
NICHD NIH HHS · T32HD07312 · United States
Databases
GENBANK
M20316
External Links
PubMed source DOI: 10.1128/mcb.8.2.778-785.1988 PMC Full Text View journal details
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