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PMID: 2833614 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequences of cDNAs for human papillomavirus type 18 transcripts in HeLa cells.

Journal of virology ·Vol. 62 ·No. 5 ·1988-05-00 ·Pages 1640-6

Inagaki Y, Tsunokawa Y, Takebe N, Nawa H, Nakanishi S, Terada M, Sugimura T

Abstract

HeLa cells expressed 3.4- and 1.6-kilobase (kb) transcripts of the integrated human papillomavirus (HPV) type 18 genome. Two types of cDNA clones representing each size of HPV type 18 transcript were isolated. Sequence analysis of these two types of cDNA clones revealed that the 3.4-kb transcript contained E6, E7, the 5' portion of E1, and human sequence and that the 1.6-kb transcript contained spliced and frameshifted E6 (E6*), E7, and human sequence. There was a common human sequence containing a poly(A) addition signal in the 3' end portions of both transcripts, indicating that they were transcribed from the HPV genome at the same integration site with different splicing. Furthermore, the 1.6-kb transcript contained both of the two viral TATA boxes upstream of E6, strongly indicating that a cellular promoter was used for its transcription.

MeSH Terms
Amino Acid Sequence Base Sequence DNA/analysis HeLa Cells/metabolism Humans Molecular Sequence Data Molecular Weight Papillomaviridae/genetics Transcription, Genetic Tumor Cells, Cultured/metabolism
Chemicals
DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Inagaki Y
National Cancer Center Research Institute, Tokyo, Japan.
Tsunokawa Y
Takebe N
Nawa H
Nakanishi S
Terada M
Sugimura T
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.62.5.1640-1646.1988
Published
1988-05-00
Pages
1640-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC253192
Subset
IM
Databases
GENBANK
M20324, M20325
External Links
PubMed source DOI: 10.1128/JVI.62.5.1640-1646.1988 PMC Full Text View journal details
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