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PMID: 3029760 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Papillomavirus sequences integrate near cellular oncogenes in some cervical carcinomas.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 4 ·1987-02-00 ·Pages 1070-4

Dürst M, Croce CM, Gissmann L, Schwarz E, Huebner K

Abstract

The chromosomal locations of cellular sequences flanking integrated papillomavirus DNA in four cervical carcinoma cell lines and a primary cervical carcinoma have been determined. The two human papillomavirus (HPV) 16 flanking sequences derived from the tumor were localized to chromosome regions 20pter----20q13 and 3p25----3qter, regions that also contain the protooncogenes c-src-1 and c-raf-1, respectively. The HPV 16 integration site in the SiHa cervical carcinoma-derived cell line is in chromosome region 13q14----13q32. The HPV 18 integration site in SW756 cervical carcinoma cells is in chromosome 12 but is not closely linked to the Ki-ras2 gene. Finally, in two cervical carcinoma cell lines, HeLa and C4-I, HPV 18 DNA is integrated in chromosome 8, 5' of the c-myc gene. The HeLa HPV 18 integration site is within 40 kilobases 5' of the c-myc gene, inside the HL60 amplification unit surrounding and including the c-myc gene. Additionally, steady-state levels of c-myc mRNA are elevated in HeLa and C4-I cells relative to other cervical carcinoma cell lines. Thus, in at least some genital tumors, cis-activation of cellular oncogenes by HPV may be involved in malignant transformation of cervical cells.

MeSH Terms
Attachment Sites, Microbiological Base Sequence Carcinoma/genetics,pathology Cell Line Chromosome Mapping Chromosomes, Human, Pair 8 Cloning, Molecular Female Gene Expression Regulation Humans Oncogenes Papillomaviridae/genetics Uterine Cervical Neoplasms/genetics,pathology
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Dürst M
Croce C M
Gissmann L
Schwarz E
Huebner K
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41 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.4.1070
Published
1987-02-00
Pages
1070-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC304363
Subset
IM
Grants
NCI NIH HHS · CA10815 · United States
NCI NIH HHS · CA21124 · United States
NCI NIH HHS · CA39860 · United States
External Links
PubMed source DOI: 10.1073/pnas.84.4.1070 PMC Full Text View journal details
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