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PMID: 283389 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Circular dichroism analysis of mononucleosome DNA conformation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 10 ·1978-10-00 ·Pages 4759-63

Cowman MK, Fasman GD

Abstract

Mononucleosomes were isolated from micrococcal nuclease digests of chicken erythrocyte nuclei. The circular dichroism properties of mononucleosome preparations, differing in average DNA length and in H1 and H5 content, demonstrate that the spectrum of chromatin is due only to the complete structure of its repeating subunits. The nucleoprotein spectra are all altered relative to protein-free DNA by the emergence of a single negative band at 275 nm, similar to the band observed for psi DNA. The intensity of the psi-type band depends on the proportion of DNA condensed in a specific manner. The psi-type band is proposed to be due to the compact DNA tertiary structure; i.e., the manner in which the DNA is wound around the histone core allowing interactions between adjacent turns of the superhelix. This interpretation attributes changes and variability in nucleoprotein circular dichroism spectra under different experimental conditions to alterations in DNA tertiary structure rather than secondary structure.

MeSH Terms
Chromatin/analysis Circular Dichroism DNA Histones Molecular Conformation
Chemicals
Chromatin Histones DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cowman M K
Fasman G D
References (34)
34 references, click to expand
  1. Influence of histone H1 on chromatin structure.
    Cell. 1977 Sep;12(1):101-7 PMID: 561660
  2. Form of DNA and the nature of interactions with proteins in chromatin.
    Nucleic Acids Res. 1978 Mar;5(3):835-50 PMID: 643619
  3. Superstructure and CD spectrum as probes of chromatin integrity.
    Nucleic Acids Res. 1978 Feb;5(2):523-35 PMID: 634797
  4. Isolation and characterization of a spacerless dinucleosome from H1-deleted chromatin.
    Nucleic Acids Res. 1977 Dec;4(12):4077-89 PMID: 600791
  5. Reconstitution of chromatin core particles.
    Biochemistry. 1977 Nov 29;16(24):5295-303 PMID: 921932
  6. Structure of nucleosome core particles of chromatin.
    Nature. 1977 Sep 1;269(5623):29-36 PMID: 895884
  7. Studies on the role of histones HI (f1) and H5 (f2c) in chromatin structure.
    Exp Cell Res. 1976 Sep;101(2):396-410 PMID: 986949
  8. Preparation and physical characterization of a homogeneous population of monomeric nucleosomes from HeLa cells.
    Nucleic Acids Res. 1976 Sep;3(9):2255-66 PMID: 967693
  9. Chromatin and nucleosome structure.
    Nucleic Acids Res. 1976 Aug;3(8):1839-55 PMID: 967679
  10. Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis.
    Biochemistry. 1967 Jun;6(6):1818-27 PMID: 6035921
  11. The secondary structure of DNA in solution and in nucleohistone.
    J Mol Biol. 1971 May 28;58(1):277-88 PMID: 5104368
  12. Spheroid chromatin units (v bodies).
    Science. 1974 Jan 25;183(4122):330-2 PMID: 4128918
  13. Structure and circular dichroism of DNA in concentrated polymer solutions.
    Nat New Biol. 1972 Mar 22;236(64):67-70 PMID: 4502454
  14. The structure of psi DNA.
    J Mol Biol. 1974 Mar 25;84(1):37-64 PMID: 4208607
  15. Studies on the structure of metaphase and interphase chromatin of Chinese hamster cells by circular dichroism and thermal denaturation.
    Biochemistry. 1972 Dec 5;11(25):4811-7 PMID: 4632121
  16. Analysis of repeating DNA sequences by reassociation.
    Methods Enzymol. 1974;29:363-418 PMID: 4850571
  17. Thermal denaturation of DNA and DNA:polypeptide complexes. Simultaneous absorption and circular dichroism measurements.
    Biochem Biophys Res Commun. 1974 Jul 24;59(2):672-9 PMID: 4859561
  18. An approach to the structure of native nucleohistone.
    Biochemistry. 1973 Jan 30;12(3):468-74 PMID: 4734233
  19. Electron microscopy and circular dichroism studies on chromatin.
    Biochemistry. 1972 Aug 1;11(16):3044-54 PMID: 5041909
  20. Circular dichroism spectra of oriented and unoriented deoxyribonucleic acid films--a preliminary study.
    J Mol Biol. 1970 Sep 28;52(3):521-41 PMID: 4923749
  21. The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.
    Biochem J. 1967 Jan;102(1):251-7 PMID: 5339944
  22. Solenoidal model for superstructure in chromatin.
    Proc Natl Acad Sci U S A. 1976 Jun;73(6):1897-901 PMID: 1064861
  23. Analysis of subunit organization in chicken erythrocyte chromatin.
    Proc Natl Acad Sci U S A. 1976 Feb;73(2):505-9 PMID: 1061151
  24. DNA structure in sheared and unsheared chromatin.
    Science. 1976 May 21;192(4241):796-8 PMID: 1265482
  25. Configuration of unsheared nucleohistone. Effects of ionic strength and of histone F1 removal.
    Biochemistry. 1976 Jan 13;15(1):186-92 PMID: 1247505
  26. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.
    Biochemistry. 1975 Aug 26;14(17):3787-94 PMID: 1174504
  27. Conformation of deoxyribonucleic acid in chromatin: a circular dichroism study.
    J Mol Biol. 1970 Aug 28;52(1):125-9 PMID: 5530396
  28. Structure of nucleic acid-poly base complexes.
    Biochemistry. 1970 Oct 27;9(22):4410-6 PMID: 5528747
  29. Conformational changes associated with f-1 histone-deoxyribonucleic acid complexes. Circular dichroism studies.
    Biochemistry. 1970 Jul 7;9(14):2814-22 PMID: 5459534
  30. Dehydrated circular DNA: circular dichroism of molecules in ethanolic solutions.
    Biopolymers. 1978 Jan;17(1):145-57 PMID: 623879
  31. Chromatin.
    Nature. 1978 Jan 12;271(5641):115-22 PMID: 340956
  32. Structure of chromatin.
    Annu Rev Biochem. 1977;46:931-54 PMID: 332067
  33. Compact oligomers and nucleosome phasing.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3583-7 PMID: 278974
  34. Involvement of histone H1 in the organization of the chromosome fiber.
    Proc Natl Acad Sci U S A. 1977 May;74(5):1879-83 PMID: 266711
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.10.4759
Published
1978-10-00
Pages
4759-63
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC336199
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.10.4759 PMC Full Text View journal details
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