Form of DNA and the nature of interactions with proteins in chromatin.

Nucleic acids research ·Vol. 5 ·No. 3 ·1978-03-00 ·Pages 835-50

Goodwin DC, Brahms J

Abstract

Studies of native chromatins and of isolated nucleosomes (from calf thymus) show that the DNA is in the B form or modified B form. This was determined by Raman spectroscopy of chromatins, of nucleosomes (from calf thymus) and of DNA fibres and directly correlated with X-ray diffraction studies. The Raman spectra of three forms of DNA (A, B and C) have been characterized in fibres both by X-ray diffraction and Raman spectroscopy on the same sample. In particular, the Raman spectrum of the C form of DNA is characterized by a band of about 870 cm(-1). For the first time, chromatins of different origins with increasing content of non-histone proteins have been investigated by Raman spectroscopy. The site of interaction of the non-histone proteins appears to involve the N7 position of guanine while the histone core does not interact at this site. It is proposed that the mechanism of specific recognition in chromatin involves the large groove.

MeSH Terms

Animals Cattle Cell Line Chromatin/ultrastructure Chromosomal Proteins, Non-Histone/metabolism DNA/metabolism DNA, Neoplasm Nucleic Acid Conformation Protein Binding Spectrum Analysis, Raman Thymus Gland X-Ray Diffraction

Chemicals

Chromatin Chromosomal Proteins, Non-Histone DNA, Neoplasm DNA

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Goodwin D C

Brahms J

References (34)

34 references, click to expand

Structure of chromatin.
Annu Rev Biochem. 1977;46:931-54 PMID: 332067
Secondary structural complementarity between DNA and proteins.
Proc Natl Acad Sci U S A. 1977 Apr;74(4):1458-62 PMID: 266187
The structure of the chromatin core particle in solution.
Nucleic Acids Res. 1977 Sep;4(9):3199-214 PMID: 561952
Secondary structure of histones and DNA in chromatin.
Science. 1977 Jul 22;197(4301):385-8 PMID: 560060
Structure of nucleosome core particles of chromatin.
Nature. 1977 Sep 1;269(5623):29-36 PMID: 895884
Infrared linear dichroism investigations of deoxyribonucleic complexes with histones H2B and H3.
Biochemistry. 1977 Jul 12;16(14):3262-6 PMID: 889799
Organization of DNA in chromatin.
Proc Natl Acad Sci U S A. 1976 Sep;73(9):3068-72 PMID: 1067602
Chromosomal proteins and chromatin structure.
Annu Rev Biochem. 1975;44:725-74 PMID: 1094921
The secondary structure of DNA in solution and in nucleohistone.
J Mol Biol. 1971 May 28;58(1):277-88 PMID: 5104368
Spheroid chromatin units (v bodies).
Science. 1974 Jan 25;183(4122):330-2 PMID: 4128918
The effect of heparin on the structure and template properties of chromatin.
FEBS Lett. 1974 Jun 1;42(2):149-53 PMID: 4854758
Transcription of chromatin by bacterial RNA polymerase.
J Mol Biol. 1973 Oct 25;80(2):229-41 PMID: 4587403
Dependence of B-A conformational change in DNA on base composition.
Nat New Biol. 1972 Mar 29;236(65):99-100 PMID: 4554380
A new approach to the characterization of the B and A forms of DNA by I.R. spectroscopy.
Biochem Biophys Res Commun. 1973 Dec 10;55(3):881-7 PMID: 4202551
How lac repressor binds to DNA.
Nature. 1972 Jun 9;237(5354):322-7 PMID: 4557395
A proposed model for interaction of polypeptides with RNA.
Proc Natl Acad Sci U S A. 1974 Feb;71(2):283-7 PMID: 4521801
Chromatographic separation of chromatin subunits.
Biochem Biophys Res Commun. 1974 Dec 23;61(4):1193-8 PMID: 4455255
The effect of trypsin on nuclease-resistant chromatin fragments.
J Biol Chem. 1974 Jan 10;249(1):152-6 PMID: 4855624
Conformational analysis of DNA-basic polypeptide complexes: possible models of nucleoprotamines and nucleohistones.
Biopolymers. 1974;13(2):313-26 PMID: 4820064
Chromatin structure: a repeating unit of histones and DNA.
Science. 1974 May 24;184(4139):868-71 PMID: 4825889
Properties of nuclease-resistant fragments of calf thymus chromatin.
J Biol Chem. 1973 Feb 10;248(3):1080-3 PMID: 4684705
Kinky helix.
Nature. 1975 Jun 12;255(5509):530-3 PMID: 1095931
Conformational prediction and circular dichroism studies on the lac repressor.
J Mol Biol. 1975 Jul 25;96(1):29-45 PMID: 1099214
Solenoidal model for superstructure in chromatin.
Proc Natl Acad Sci U S A. 1976 Jun;73(6):1897-901 PMID: 1064861
Sequence-specific recognition of double helical nucleic acids by proteins.
Proc Natl Acad Sci U S A. 1976 Mar;73(3):804-8 PMID: 1062791
Laser Raman identification of an interaction site on DNA for arginine containing histones in chromatin.
Biochem Biophys Res Commun. 1976 Feb 23;68(4):1242-7 PMID: 1267777
A model for the binding of lac repressor to the lac operator.
Mol Biol Rep. 1976 Apr;2(5):427-34 PMID: 1272266
Detection of the sites of alkylation in DNA and polynucleotides by laser Raman spectroscopy.
Biochemistry. 1976 Jun 15;15(12):2650-5 PMID: 938635
Structural investigations on DNA-protamine complexes.
Biopolymers. 1976 Apr;15(4):687-706 PMID: 1252603
A comparison of the digestion of nuclei and chromatin by staphylococcal nuclease.
Biochemistry. 1975 Jul;14(13):2915-20 PMID: 1148184
Conformational geometry and vibrational frequencies of nucleic acid chains.
Biopolymers. 1975 Jun;14(6):1259-71 PMID: 1164547
Electron microscopic and biochemical evidence that chromatin structure is a repeating unit.
Cell. 1975 Apr;4(4):281-300 PMID: 1122558
Molecular and crystal structures of double-helical RNA. I. An x-ray diffraction study of fragmented yeast RNA and a preliminary double-helical RNA model.
J Mol Biol. 1967 Aug 14;27(3):507-24 PMID: 6058224
Internal structure of the chromatin subunit.
Nucleic Acids Res. 1974 Nov;1(11):1573-8 PMID: 10793712

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/5.3.835

Published

1978-03-00

Pages

835-50

Language

English

Region

England

NLM ID

0411011

PMCID

PMC342027

Subset

External Links

PubMed source DOI: 10.1093/nar/5.3.835 PMC Full Text View journal details

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Form of DNA and the nature of interactions with proteins in chromatin.

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