Synthesis of decadeoxyribonucleotides containing 5-modified uracils and their interactions with restriction endonucleases Bgl II, Sau 3AI and Mbo I (nucleosides and nucleotides 82).

Nucleic acids research ·Vol. 16 ·No. 11 ·1988-06-10 ·Pages 4761-76

Hayakawa T, Ono A, Ueda T

Abstract

Decadeoxyribonucleotides containing uracil, 5-bromouracil, 5-cyanouracil and 5-ethyluracil in recognition sequences of restriction endonucleases Bgl II, Sau 3AI, Mbo I were synthesized. Decanucleotides containing 5-bromouracil in place of thymine had essentially the same susceptibility to all the restriction endonucleases. Uracil-containing decanucleotides were however very resistant to attack. Decanucleotides containing 5-cyanouracil in the recognition sequence were strongly resistant to hydrolysis by Sau 3AI, but were hydrolysed by Bgl II and Mbo I as well as the parent decanucleotide. Decanucleotides containing 5-ethyluracil were strongly resistant to hydrolysis by Sau 3AI, but were partially resistant to hydrolysis by Bgl II and Mbo I.

MeSH Terms

Bacterial Proteins Bromouracil Circular Dichroism DNA Restriction Enzymes/metabolism Deoxyribonucleases, Type II Site-Specific Deoxyribonucleotides/chemical synthesis,metabolism Structure-Activity Relationship Substrate Specificity Uracil/analogs & derivatives

Chemicals

Bacterial Proteins Deoxyribonucleotides 5-ethyluracil 5-cyanouracil Bromouracil Uracil DNA Restriction Enzymes BglII endonuclease Deoxyribonucleases, Type II Site-Specific GATC-specific type II deoxyribonucleases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Hayakawa T

Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.

Ono A

Ueda T

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22 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/16.11.4761

Published

1988-06-10

Pages

4761-76

Language

English

Region

England

NLM ID

0411011

PMCID

PMC336694

Subset

External Links

PubMed source DOI: 10.1093/nar/16.11.4761 PMC Full Text View journal details

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Synthesis of decadeoxyribonucleotides containing 5-modified uracils and their interactions with restriction endonucleases Bgl II, Sau 3AI and Mbo I (nucleosides and nucleotides 82).

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