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PMID: 2997733 Published · ppublish English Journal Article

Interaction of the restriction endonuclease ScaI with its substrates.

Nucleic acids research ·Vol. 13 ·No. 19 ·1985-10-11 ·Pages 7015-24

Kita K, Hiraoka N, Kimizuka F, Obayashi A, Kojima H, Takahashi H, Saito H

Abstract

The kinetic constants of the site-specific endonuclease, ScaI, for various substrates were determined. We estimated Vmax and Km for octa-, deca-, dodeca-, and hexadecanucleotides and for plasmid pBR322 DNA. Vmax for these substrates were close, but Km were quite different (in decreasing order, octa- greater than deca-, dodeca-, hexadeca- greater than pBR322). The results were discussed with respect to the tertiary structure of substrate.

MeSH Terms
DNA Restriction Enzymes/metabolism Deoxyribonucleases, Type II Site-Specific Kinetics Nucleic Acid Conformation Oligodeoxyribonucleotides/chemical synthesis,metabolism Plasmids Streptomyces/enzymology Substrate Specificity
Chemicals
Oligodeoxyribonucleotides DNA Restriction Enzymes endodeoxyribonuclease ScaI Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Kita K
Hiraoka N
Kimizuka F
Obayashi A
Kojima H
Takahashi H
Saito H
References (19)
19 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.19.7015
Published
1985-10-11
Pages
7015-24
Language
English
Region
England
NLM ID
0411011
PMCID
PMC322019
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/13.19.7015 PMC Full Text View journal details
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