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PMID: 2839701 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation and characterization of the genes for two small RNAs of herpesvirus papio and their comparison with Epstein-Barr virus-encoded EBER RNAs.

Journal of virology ·Vol. 62 ·No. 8 ·1988-08-00 ·Pages 2790-8

Howe JG, Shu MD

Abstract

Genes for the Epstein-Barr virus-encoded RNAs (EBERs), two low-molecular-weight RNAs encoded by the human gammaherpesvirus Epstein-Barr virus (EBV), hybridize to two small RNAs in a baboon cell line that contains a similar virus, herpesvirus papio (HVP). The genes for the HVP RNAs (HVP-1 and HVP-2) are located together in the small unique region at the left end of the viral genome and are transcribed by RNA polymerase III in a rightward direction, similar to the EBERs. There is significant similarity between EBER1 and HVP-1 RNA, except for an insert of 22 nucleotides which increases the length of HVP-1 RNA to 190 nucleotides. There is less similarity between the sequences of EBER2 and HVP-2 RNA, but both have a length of about 170 nucleotides. The predicted secondary structure of each HVP RNA is remarkably similar to that of the respective EBER, implying that the secondary structures are important for function. Upstream from the initiation sites of all four RNA genes are several highly conserved sequences which may function in the regulation of transcription. The HVP RNAs, together with the EBERs, are highly abundant in transformed cells and are efficiently bound by the cellular La protein.

MeSH Terms
Autoantigens/metabolism Base Sequence Cloning, Molecular Genes, Viral Herpesviridae/genetics Herpesvirus 4, Human/genetics Hydrogen Bonding Molecular Sequence Data Nucleic Acid Conformation RNA Polymerase III/metabolism RNA, Viral/genetics Ribonucleoproteins/genetics Structure-Activity Relationship Transcription, Genetic
Chemicals
Autoantigens RNA, Viral Ribonucleoproteins SS-B antigen RNA Polymerase III
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Howe J G
Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06510-8024.
Shu M D
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.62.8.2790-2798.1988
Published
1988-08-00
Pages
2790-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC253713
Subset
IM
Grants
NCI NIH HHS · CA 16038-14 · United States
Databases
GENBANK
M20563
External Links
PubMed source DOI: 10.1128/JVI.62.8.2790-2798.1988 PMC Full Text View journal details
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