Home LiteratureArticle Details
PMID: 2841644 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

IS150: distribution, nucleotide sequence and phylogenetic relationships of a new E. coli insertion element.

Nucleic acids research ·Vol. 16 ·No. 14B ·1988-07-25 ·Pages 6789-802

Schwartz E, Kröger M, Rak B

Abstract

Recently we identified the new insertion (IS) sequence IS150 in various strains of Escherichia coli K-12. We have screened other strains of E. coli and Salmonella typhimurium for the presence of homologous sequences. The strains of E. coli K-12 and W tested contain one or more copies of homology to IS150. We have also determined the complete nucleotide sequence of a copy of IS150 inserted into IS1. Comparison of nucleotide and deduced amino acid sequences of IS150, IS2, IS3, IS51, IS600 and IS629 reveals significant homologies suggesting that these elements are members of a family of phylogenetically related insertion sequences.

MeSH Terms
Amino Acid Sequence Base Sequence DNA Transposable Elements Escherichia coli/genetics Genes, Bacterial Molecular Sequence Data Phylogeny Promoter Regions, Genetic Sequence Homology, Nucleic Acid
Chemicals
DNA Transposable Elements
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Schwartz E
Institut für Biologie III, Universität Freiburg, FRG.
Kröger M
Rak B
References (31)
31 references, click to expand
  1. Insertion of DNA activates the cryptic bgl operon in E. coli K12.
    Nature. 1981 Oct 22;293(5834):625-9 PMID: 6270569
  2. Method to determine the reading frame of a protein from the purine/pyrimidine genome sequence and its possible evolutionary justification.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1596-600 PMID: 6940175
  3. Transposable elements in prokaryotes.
    Annu Rev Genet. 1981;15:341-404 PMID: 6279020
  4. IS2 insertion is a major cause of spontaneous mutagenesis of the bacteriophage P1: non-random distribution of target sites.
    EMBO J. 1983;2(1):67-71 PMID: 11894911
  5. Expression of two proteins from overlapping and oppositely oriented genes on transposable DNA insertion element IS5.
    Nature. 1982 May 13;297(5862):124-8 PMID: 6281651
  6. Simplified computer programs for search of homology within nucleotide sequences.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):193-201 PMID: 6546417
  7. Nucleotide sequence of insertion sequence IS3411, which flanks the citrate utilization determinant of transposon Tn3411.
    J Bacteriol. 1988 Apr;170(4):1902-6 PMID: 2832386
  8. Insertion sequence elements of Pseudomonas savastanoi: Nucleotide sequence and homology with Agrobacterium tumefaciens transfer DNA.
    Proc Natl Acad Sci U S A. 1986 Nov;83(21):8263-7 PMID: 16593778
  9. Analysis of E. coli promoter sequences.
    Nucleic Acids Res. 1987 Mar 11;15(5):2343-61 PMID: 3550697
  10. IS3 can function as a mobile promoter in E. coli.
    Nucleic Acids Res. 1982 Oct 11;10(19):5935-48 PMID: 6292860
  11. Expression, purification and operator binding of the transposon Tn1721-encoded Tet repressor.
    J Mol Biol. 1986 Jun 20;189(4):633-41 PMID: 2431153
  12. Characterization and properties of very large inversions of the E. coli chromosome along the origin-to-terminus axis.
    Mol Gen Genet. 1985;201(3):467-76 PMID: 3911026
  13. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  14. Insertion of IS2 creates a novel ampC promoter in Escherichia coli.
    Cell. 1983 Mar;32(3):809-16 PMID: 6187472
  15. A chain of interlinked genes in the ninR region of bacteriophage lambda.
    Gene. 1982 Nov;20(1):25-38 PMID: 6219042
  16. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  17. Beta-glucoside (bgl) operon of Escherichia coli K-12: nucleotide sequence, genetic organization, and possible evolutionary relationship to regulatory components of two Bacillus subtilis genes.
    J Bacteriol. 1987 Jun;169(6):2579-90 PMID: 3034860
  18. Genesis and natural history of IS-mediated transposons.
    Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:27-43 PMID: 6271475
  19. Genetic organization of insertion element IS2 based on a revised nucleotide sequence.
    Gene. 1987;59(2-3):291-6 PMID: 2830172
  20. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
    J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
  21. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
  22. The bidirectional transfer of DNA and RNA to nitrocellulose or diazobenzyloxymethyl-paper.
    Anal Biochem. 1980 Nov 15;109(1):123-9 PMID: 6162401
  23. A general method applicable to the search for similarities in the amino acid sequence of two proteins.
    J Mol Biol. 1970 Mar;48(3):443-53 PMID: 5420325
  24. Autoregulation and function of a repressor in bacteriophage lambda.
    Science. 1976 Oct 8;194(4261):156-61 PMID: 959843
  25. IS2, a genetic element for turn-off and turn-on of gene activity in E. coli.
    Mol Gen Genet. 1974;132(4):265-89 PMID: 4610339
  26. Insertion element IS5 contains a third gene.
    EMBO J. 1984 Apr;3(4):807-11 PMID: 6327289
  27. Complete sequence of IS3.
    Nucleic Acids Res. 1985 Mar 25;13(6):2127-39 PMID: 2987844
  28. Isolation and characterization of IS elements repeated in the bacterial chromosome.
    J Mol Biol. 1987 Aug 5;196(3):445-55 PMID: 2824781
  29. Second-element turn-on of gene expression in an IS1 insertion mutant.
    Mol Gen Genet. 1988 Feb;211(2):282-9 PMID: 2832704
  30. Chromosome transfer and Hfr formation by F in rec+ and recA strains of Escherichia coli K12.
    Plasmid. 1979 Jul;2(3):358-65 PMID: 384417
  31. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.14.6789
Published
1988-07-25
Pages
6789-802
Language
English
Region
England
NLM ID
0411011
PMCID
PMC338333
Subset
IM
Databases
GENBANK
X07037
External Links
PubMed source DOI: 10.1093/nar/16.14.6789 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2