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PMID: 2987844 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Complete sequence of IS3.

Nucleic acids research ·Vol. 13 ·No. 6 ·1985-03-25 ·Pages 2127-39

Timmerman KP, Tu CP

Abstract

We have determined the nucleotide sequence of IS3. Our IS3 isolate has 39 bp inverted repeats (IR's) with 6 mismatches, and is 1258 bp long. IS3 contains a large open reading frame (ORF) of 288 codons with a smaller, partially overlapping ORF of 91 codons on the opposite strand in codon-codon register. The large ORF is preceded by and has a 4 bp overlap with a 99 codon ORF that has potential transcriptional and translational start signals. Thus, IS3 could encode a bicistronic mRNA. The Shine-Dalgarno sequence for this 99 codon ORF could be sequestered in a stem-loop structure, but only if the transcript began outside IS3, as was first seen with IS10. This could be a means for preventing fortuitous activation of IS3 by outside promoters. No DNA sequence homology was found between IS3 and other prokaryotic IS elements, but there is slight amino acid sequence homology and significant conservation of hydropathicity patterns between the putative transposases of IS3 and IS2.

MeSH Terms
Base Sequence DNA Transposable Elements DNA, Bacterial/genetics Escherichia coli/genetics Genes Nucleotidyltransferases/genetics Solubility Transposases
Chemicals
DNA Transposable Elements DNA, Bacterial Nucleotidyltransferases Transposases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Timmerman K P
Tu C P
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51 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.6.2127
Published
1985-03-25
Pages
2127-39
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341140
Subset
IM
Databases
GENBANK
X02311
External Links
PubMed source DOI: 10.1093/nar/13.6.2127 PMC Full Text View journal details
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