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PMID: 2832704 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Second-element turn-on of gene expression in an IS1 insertion mutant.

Molecular & general genetics : MGG ·Vol. 211 ·No. 2 ·1988-02-00 ·Pages 282-9

Schwartz E, Herberger C, Rak B

Abstract

To learn more about the ways in which genes silenced by insertion mutations can be reactivated, we have undertaken a systematic investigation of Gal+ revertants of the polar mutant galOP-306::IS1 in Escherichia coli K12. The selective conditions used excluded reversion to wild type by precise excision of IS1. In this system (which resided on a multi-copy plasmid) reversion to the Gal+ phenotype occurred with a frequency of about 10(-7) per cell and per generation. Analysis of the revertants revealed that - with the single exception of the previously published chromosomal mutant sis1 - alterations in the structure of IS1 lead to reactivation of gal operon expression. These events fall into four classes: (I) insertion of IS2 at position 327 in IS1, insertion of IS2 at position 687 in IS1, (III) insertion of a hitherto undetected mobile element, IS150, at position 387, (IV) a 16-bp deletion encompassing IS1 coordinates 553-568. Of some 200 independent reversion events studied, all but one were of types I-III i.e. they involved the intervention of a second mobile element.

MeSH Terms
Base Sequence DNA Restriction Enzymes DNA Transposable Elements Escherichia coli/genetics Gene Expression Regulation Genes, Bacterial Molecular Sequence Data Mutation Plasmids
Chemicals
DNA Transposable Elements DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Schwartz E
Institut für Biologie III, Universität Freiburg, Federal Republic of Germany.
Herberger C
Rak B
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00330605
Published
1988-02-00
Pages
282-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00330605
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