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PMID: 6316112 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

A control system which causes alternating turn-off/turn-on of transcription on insertion element IS1.

Molecular & general genetics : MGG ·Vol. 192 ·No. 1-2 ·1983-00-00 ·Pages 61-5

Rak B

Abstract

Presence of insertion element IS1 within an operon leads to absence of expression of genes distal to its integration site. This strong polar effect is observed irrespective of the orientation of IS1. Here I report on a mutant of E. coli K12 which allows turn-on of genes located distally to IS1. This turn-on is due to activation of transcription starting within IS1. The activation of transcription is under the control of a regulatory locus (sis1) which acts in trans. The regulatory locus itself changes its state, which leads to an alternating turn-off/turn-on of genes located distal to IS1: The rate of turn-off was observed with a frequency of about 0.3% (per cell and per generation), that of turn-on with a frequency of about 0.015% (per cell and per generation).

MeSH Terms
DNA Transposable Elements Escherichia coli/genetics Genes, Bacterial Mutation Operon Phenotype Plasmids Suppression, Genetic Transcription, Genetic
Chemicals
DNA Transposable Elements
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Rak B
References (31)
31 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00327647
Published
1983-00-00
Pages
61-5
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00327647
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