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PMID: 2850471 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The coordinate replication of the human beta-globin gene domain reflects its transcriptional activity and nuclease hypersensitivity.

Molecular and cellular biology ·Vol. 8 ·No. 11 ·1988-11-00 ·Pages 4958-65

Dhar V, Mager D, Iqbal A, Schildkraut CL

Abstract

The temporal order of replication of DNA sequences in the chromosomal domain containing the human beta-globin gene cluster and its flanking sequences (140 kilobases) was measured and compared in two different human cell lines. In human erythroleukemia (K562) cells, in which embryonic and fetal globin genes are transcribed, all of the sequences we examined from the beta-globin domain replicated early during S phase, while in HeLa cells, in which globin genes are transcriptionally silent, these sequences replicated late during S. Potential sites of initiation of DNA replication within this domain were identified. The beta-globin gene domain was also found to differ with respect to the nuclease sensitivity of the chromatin in these two cell lines. In K562 cells, hypersensitive sites for endogenous nucleases and DNase I were present in the chromatin near the earliest-replicating segments in the beta-globin domain.

MeSH Terms
DNA Replication DNA Restriction Enzymes Deoxyribonucleases Globins/genetics HeLa Cells/metabolism Humans Leukemia, Erythroblastic, Acute/genetics Transcription, Genetic Tumor Cells, Cultured/metabolism
Chemicals
Globins Deoxyribonucleases DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Dhar V
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461.
Mager D
Iqbal A
Schildkraut C L
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45 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.11.4958-4965.1988
Published
1988-11-00
Pages
4958-65
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365589
Subset
IM
Grants
NCI NIH HHS · 5 T32 CA09060 · United States
NIGMS NIH HHS · GM20069 · United States
NCI NIH HHS · P30-CA13330 · United States
External Links
PubMed source DOI: 10.1128/mcb.8.11.4958-4965.1988 PMC Full Text View journal details
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